DSHB AIIB2 说明书

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The Developmental Studies Hybridoma Bank

  • was created by NIH as a national resource,
  • sells at cost (e.g. 1 ml of supernatant costs $40.00, not $200.00 or more),
  • is growing at 10% to 20% per year,
  • has been given the distribution rights for monoclonals being generated by an NCI initiative in diagnostics, and
  • has begun a second bank for microbes (DSHB-Microbe). 

 

AIIB2

Antigen: Integrin, beta-1 subunit Hybridoma Cells Available: Yes
Antigen Species: Human Depositor: Damsky, C.H.
Antigen Sequence: Depositors Institution: University of California, San Francisco
Antigen Molecular Weight: Predicted: 88kDa (multiple isoforms 87 to 92kDa); Apparent: 110kDa non-reduced gel mobility, 130kDa reduced gel mobility Human Protein Atlas: Link
Positive Tested Species Reactivity: Bovine, Canine, Human, Mouse, Sheep Depositors Notes: Blocks cell attachment to fibronectin, collagen-I, collagen-IV, laminin. This antibody stains aldehyde fixed cells and frozen sections of fixed tissues. Does not appear to work for immunoblotting. Binds protein G.
Gene: ITGB1 Immunogen: Whole JAR choriocarcinoma trophoblastic cell line
Alternate Gene Names: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA Immunogen Sequence: Full length protein
Isotype: RIgG1 Alternate Antibody Name:
Clonality: Monoclonal Alternate Antigen Name:
Epitope Mapped: Yes Myeloma Strain: SP 2/0
Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218) Uniprot ID: P05556
Host Species: rat Entrez Gene ID: 3688
Additional Characterization: Link Antibody Registry ID: AB_528306
Additional Information: Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited papers for protocols required for western blotting.  
Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot