细胞增殖及毒性检测试剂盒

发表于2024年4月18日由Amresco中国官网

上海金畔生物科技有限公司提供细胞增殖及毒性检测试剂盒，欢迎访问官网了解更多产品信息。

产品编号	BA00208
英文名称	Cell Counting Kit-8
中文名称	细胞增殖及毒性检测试剂盒
别名	Cell Counting Kit-8 (CCK-8); CCK-8; WST-8; Enhanced Cell Counting Kit-8; CCK-8 Cell Proliferation and Cytotoxicity Assay Kit; CCK-8 WST-8; Cell Counting Kit – 8; CCK-8细胞增殖及毒性检测试剂盒; CCK8试剂盒; CCK-8/WST-8试剂盒; CCK-8细胞活力检测试剂盒; 细胞计数试剂盒-8（CCK-8）; CCK-8试剂盒(细胞增殖及毒性检测试剂盒); Cell Counting Kit-8（CCK-8试剂盒）; 细胞计数试剂盒-8; Cell Counting Kit-8(CCK-8)细胞活性检测试剂盒; 细胞活性检测试剂盒;
	Specific References (99) \| BA00208 has been referenced in 99 publications. [IF=17.694] Hu, Jinyuan. et al. Design of synthetic collagens that assemble into supramolecular banded fibers as a functional biomaterial testbed. NAT COMMUN. 2022 Nov;13(1):1-13 PubMed:36351904 [IF=10.75] Liu, Zhenni. et al. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. INT J BIOL SCI. 2023 Jan;19(3):950-966 PubMed:36778123 [IF=10.684] Peng Luo. et al. OP3-4 peptide sustained-release hydrogel inhibits osteoclast formation and promotes vascularization to promote bone regeneration in a rat femoral defect model. BIOENG TRANSL MED. 2022 Oct;:e10414 Other ; Other. PubMed:10.1002/btm2.10414 [IF=9.685] Yang, Wenjing. et al. BTN3A1 promotes tumor progression and radiation resistance in esophageal squamous cell carcinoma by regulating ULK1-mediated autophagy. CELL DEATH DIS. 2022 Nov;13(11):1-17 PubMed:36418890 [IF=9.417] Zhenjia Che. et al. Bifunctionalized hydrogels promote angiogenesis and osseointegration at the interface of three-dimensionally printed porous titanium scaffolds. MATER DESIGN. 2022 Nov;223:111118 Other ; Other. PubMed:10.1016/j.matdes.2022.111118 [IF=9.229] Mengjia Wang. et al. Binding Peptide-Promoted Biofunctionalization of Graphene Paper with Hydroxyapatite for Stimulating Osteogenic Differentiation of Mesenchymal Stem Cells. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX PubMed:34962367 [IF=8.786] Junjun Guo. et al. TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. FRONT IMMUNOL. 2023; 14: 1114620 PubMed:37122710 [IF=8.786] Xiaogang Shen. et al. Integrating machine learning and single-cell trajectories to analyze T-cell exhaustion to predict prognosis and immunotherapy in colon cancer patients. FRONT IMMUNOL. 2023; 14: 1162843 PubMed:37207222 [IF=8.025] Gaofeng Cai. et al. Structure of a Pueraria root polysaccharide and its immunoregulatory activity on T and B lymphocytes, macrophages, and immunosuppressive mice. INT J BIOL MACROMOL. 2023 Jan;:123386 PubMed:36702224 [IF=8.013] Yuchen Liu. et al. Docosahexaenoic Acid Attenuates Radiation-Induced Myocardial Fibrosis by Inhibiting the p38/ET-1 Pathway in Cardiomyocytes. INT J RADIAT ONCOL. 2022 Dec;: PubMed:36529557 [IF=7.666] Shulipan Mulati. et al. 6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer. CELLS-BASEL. 2022 Jan;11(22):3678 PubMed:36429106 [IF=7.419] Li-Ping Yu. et al. In vivo identification of the pharmacodynamic ingredients of Polygonum cuspidatum for remedying the mitochondria to alleviate metabolic dysfunction–associated fatty liver disease. BIOMED PHARMACOTHER. 2022 Dec;156:113849 Other ; Other. PubMed:36252355 [IF=7.397] Shunjie Yu. et al. TIM3/CEACAM1 pathway involves in myeloid-derived suppressor cells induced CD8+ T cells exhaustion and bone marrow inflammatory microenvironment in myelodysplastic syndrome. IMMUNOLOGY. 2022 May 03 PubMed:35470423 [IF=7.109] Zhao, Renchang. et al. Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes. CELL DEATH DISCOV. 2023 Jan;9(1):1-10 PubMed:36635258 [IF=6.656] Gaofeng Cai. et al. The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. PHYTOMEDICINE. 2022 Aug;103:154232 PubMed:35675749 [IF=6.656] Zhenwei Zhou. et al. BuShen JianGu Fang alleviates cartilage degeneration via regulating multiple genes and signaling pathways to activate NF-κB/Sox9 axis. PHYTOMEDICINE. 2023 May;113:154742 PubMed:36893673 [IF=6.58] Luqin Wan. et al. The advanced glycation end-products (AGEs)/ROS/NLRP3 inflammasome axis contributes to delayed diabetic corneal wound healing and nerve regeneration. Int J Biol Sci. 2022; 18(2): 809–825 Other ; Other. PubMed:35002527 [IF=6.53] Shilei Zhang. et al. A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue. BIOMED PHARMACOTHER. 2022 Jun;150:113004 Other ; Other. PubMed:10.1016/j.biopha.2022.113004 [IF=6.53] Xing-Hua Xiao. et al. Magnolol alleviates hypoxia-induced pulmonary vascular remodeling through inhibition of phenotypic transformation in pulmonary arterial smooth muscle cells. BIOMED PHARMACOTHER. 2022 Jun;150:113060 PubMed:35658230 [IF=6.529] Huifeng Wang. et al. Periplocin ameliorates mouse age-related meibomian gland dysfunction through up-regulation of Na/K-ATPase via SRC pathway. Biomed Pharmacother. 2022 Feb;146:112487 PubMed:34883449 [IF=6.388] Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 Apr;: PubMed:37086182 [IF=6.306] Yu Han. et al. Structural characterization and transcript-metabolite correlation network of immunostimulatory effects of sulfated polysaccharides from green alga Ulva pertusa. Food Chem. 2021 Apr;342:128537 PubMed:33183876 [IF=6.289] Zhengqing Zhu. et al. Three-dimensionally printed porous biomimetic composite for sustained release of recombinant human bone morphogenetic protein 9 to promote osteointegration. Mater Design. 2021 Oct;208:109882 Other ; PubMed:10.1016/j.matdes.2021.109882 [IF=6.208] Lan Wang. et al. Fenbendazole Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice via Suppression of Fibroblast-to-Myofibroblast Differentiation. INT J MOL SCI. 2022 Jan;23(22):14088 PubMed:36430565 [IF=6.117] Xiaoshan Liang. et al. Folic Acid Ameliorates Synaptic Impairment following Cerebral Ischemia/Reperfusion Injury via Inhibiting Excessive Activation of NMDA Receptors. J NUTR BIOCHEM. 2022 Nov;:109209 PubMed:36370927 [IF=6.081] Min Yu. et al. RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. Biomedicines. 2021 Sep;9(9):1244 Other ; mouse. PubMed:34572430 [IF=6.081] Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936 PubMed:37283947 [IF=6.023] Huan Liu. et al. Effect of DEHP and DnOP on mitochondrial damage and related pathways of Nrf2 and SIRT1/PGC-1α in HepG2 cells. Food Chem Toxicol. 2021 Dec;158:112696 CCK8 ; PubMed:34822940 [IF=5.923] Jiali Xiong. et al. Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation. Int J Mol Sci. 2022 Jan;23(3):1413 Other ; Other. PubMed:35163336 [IF=5.846] Mengqi Gu. et al. Influence of placental exosomes from early onset preeclampsia women umbilical cord plasma on human umbilical vein endothelial cells. FRONT CARDIOVASC MED. 2022 Dec 23;9:1061340 PubMed:36620649
保存条件	Protect from light. Store at 2-8℃ for one year. Store at -20℃ for two year.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍	Cell Counting Kit-8，即增强型 CCK-8 试剂盒，是一种基于 WST-8 而广泛应用于细胞增殖和细胞毒性的快速、高灵敏度、无放射性的比色检测试剂盒。WST-8 在电子耦合试剂存在的情况下，可以被线粒体内的一些脱氢酶还原生成橙黄色的 formazan，且细胞增殖越多越快，则颜色越深；细胞毒性越大，则颜色越浅。对于同样的细胞，颜色的深浅 (生成的 formazan 量) 和细胞数目呈线性关系。 WST-8 是 MTT 的一种升级替代产品，和 MTT 或其它 MTT 类似产品，如 XTT、MTS 等相比有明显的优点。第一，MTT 被线粒体内的一些脱氢酶还原生成的 formazan 不是水溶性的，需要有特定的溶剂来溶解；而 WST-8 和 XTT、MTS 产生的 formazan 都是水溶性的，可以省去后续的溶解步骤。第二，WST-8 产生的 formazan 比 XTT 和 MTS 产生的 formazan 更易溶解。第三，WST-8 比 XTT 和 MTS更加稳定，使实验结果更可靠。第四，WST-8 和 MTT、XTT 等相比线性范围更宽，灵敏度更高，并且更加稳定。 WST-8 对细胞无明显毒性，可以直接加入到细胞样品中，无需预配各种成分；显色后，可以在不同时间反复用酶标仪读板，检测时间更加灵活，便于确定最佳测定时间；酚红和血清对本试剂盒的测定无明显影响。
产品图片	Bioss CCK-8产品(BA00208)与国际G品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际G品牌同等优质. Bioss CCK-8产品(BA00208)与国际D品牌对比图:图中左侧样本为SP2/O的检测数据,右侧样本为3T3的检测数据.Bioss产品稳定性与特异性与国际D品牌同等优质.