фX174 RF I DNA | NEB酶试剂 New England Biolabs

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ΦX174 RF I DNA

  • View sequence details
  • Commonly used cloning vector
  • Covalently closed circular form of PhiX174

фX174 RF I DNA  | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N3021S 1,000 µg/ml 30 µg ¥829.00
N3021L 1,000 µg/ml 150 µg ¥3,269.00
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产品信息

This is the double-stranded, covalently closed, circular form of ΦX174 RF I DNA (supercoiled). The molecular weight of ΦX174 RF I is 3.50 x 106 daltons and it is 5,386 base pairs in length. The vast majority of the molecules are RF I, and the remainder are RF II, as determined by agarose gel electrophoresis.

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source:

The ΦX174 RF I DNA is isolated from E. coli HF 4704 infected with ΦX174 am3 cs70 by a modification of the method of Godson and Vapnek (nucleotide sequenced by F. Sanger).

产品类别:
DNA Plasmids & Substrates Products

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分:

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N3021S     -20    
        ΦX174 RF I DNA N3021SVIAL -20 1 x 0.03 ml 1 mg/ml
    • N3021L     -20    
        ΦX174 RF I DNA N3021LVIAL -20 1 x 0.15 ml 1 mg/ml

  • 特性和用法

    贮存溶液

    10 mM Tris-HCl
    1 mM EDTA
    pH 8 @ 25°C

    序列文件

    GenBank

  • 相关产品

    相关产品

    • фX174 Virion DNA
    • фX174 RF II DNA
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

  • 参考文献
    1. Sanger, F. et al. (1978). J. Mol. Biol.. 125, 225-246.
    2. Godson, G.N. and Vapnek, D. (1973). Biochem.Biophys. Acta. 299, 516.

工具 & 资源

  • Web 工具
    • DNA Sequences and Maps Tool

FAQs & 问题解决指南

  • FAQs
    1. How homogeneous is the DNA/ how much is supercoiled?
    2. Are the plasmids cesium purified?
    3. What is the DNA sequence of this product?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    фX174 RF I DNA  | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
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  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]

    • N3021S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]

    • N3021S_L_v1_1771401
    • N3021S_L_v1_1771407
    • N3021S_L_v1_1791707
    • N3021S_L_v1_1781612
    • N3021S_L_v1_1781704
    • N3021S_L_v1_1791712
    • N3021L_v1_10009674
    • N3021S_v1_10008162
    • N3021L_v1_10021331
    • N3021S_v1_10021333
    • N3021S_v1_10025704
    • N3021L_v1_10025701
    • N3021S_v1_10050295
    • N3021L_v1_10055012
    • N3021L_v1_10063437
    • N3021S_v1_10068017
    • N3021L_v1_10073972
    • N3021L_v1_10084395
    • N3021S_v1_10086399
    • N3021S_v1_10096540
    • N3021L_v1_10100834
    • N3021S_v1_10100831
    • N3021L_v1_10117562
    • N3021S_v1_10117564
    • N3021L_v1_10139906
    • N3021S_v1_10139905
    • N3021S_v1_10161038
    • N3021S_v1_10163016
    • N3021L_v1_10161040

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • ΦX174 RF I DNA