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Klenow Fragment (3’→5′ exo-)
DNA Pol I, Large (Klenow) fragment was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3′ —> 5’ exonuclease activity.
- Generates probes using random primers
- Dideoxy sequencing
- Random priming labeling
- Moderate strand displacement activity
- Not suitable for generating blunt ends
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