上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Klenow Fragment (3’→5′ exo-)
DNA Pol I, Large (Klenow) fragment was originally derived as a proteolytic product of E. coli DNA polymerase. It retains polymerase activity, but lacks both 5’ —> 3’ and 3′ —> 5’ exonuclease activity.
- Generates probes using random primers
- Dideoxy sequencing
- Random priming labeling
- Moderate strand displacement activity
- Not suitable for generating blunt ends
Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders.
Learn More
Streptavidin |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Streptavidin
- Streptavidin is a 52.8 kDa non-glycosylated protein with a very high affinity for biotin
- Due to its high affinity for biotin, it can be used to bridge biotinylated probes and biotinylated enzymes
Anti-M13 pIII Monoclonal Antibody |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Anti-M13 pIII Monoclonal Antibody
- Catalog # E8033 was discontinued on September 15, 2022
Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes non-reduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage is not recommended as the epitope lies in a region not accessible on intact M13 phage virions.
- Can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis
- Recommended dilution for Western blot: 1:1000
Storage Temperature: -20°C
New england biolabs品牌代理商
上海金畔生物科技有限公司是New england biolabs品牌代理商 ，欢迎访问官网了解更多产品信息和订购。
New england biolabs
NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂。目前，NEB为基因组研究提供的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。
NEB公司成立于二十世纪七十年代中期，拥有众多经验丰富的科学家，是生产生命科学试剂。目前，NEB为基因组研究提供的重组酶和天然酶，并且公司业务范围已延伸至蛋白质组学和药品开发领域。
Founded in the mid-1970s by scientists committed to developing innovative products for the life sciences, New England Biolabs is a recognized world leader in the discovery, development and commercialization of reagents for genomic research.
产品列表：
|
No. |
品牌 |
货号 |
名称 |
规格 |
|
1 |
New england biolabs |
E6350S |
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads |
6 reactions |
|
2 |
New england biolabs |
M0492S |
Q5® High-Fidelity 2X Master Mix |
100 reactions |
|
3 |
New england biolabs |
M0205S |
E. coli DNA Ligase |
200 units |
|
4 |
New england biolabs |
R0734S |
Esp3I |
300 units |
|
5 |
New england biolabs |
T1020S |
Monarch® DNA Gel Extraction Kit |
50 preps |
|
6 |
New england biolabs |
E0546S |
BioBrick® Assembly Kit |
50 reactions |
|
7 |
New england biolabs |
M0386S |
Cas9 Nuclease, S. pyogenes |
70 pmol |
|
8 |
New england biolabs |
C1010S |
Cloning Competent E.coli Sampler |
8 tubes |
|
9 |
New england biolabs |
N3233S |
Low Molecular Weight DNA Ladder |
0.1 mL |
|
10 |
New england biolabs |
E1610S |
EpiMark® N6-Methyladenosine Enrichment Kit |
20 reactions |
|
11 |
New england biolabs |
E9100S |
SNAP-Cell® Starter Kit |
1 set |
|
12 |
New england biolabs |
P0763S |
E. coli Ribosome |
1 mg |
|
13 |
New england biolabs |
M0649S |
Nucleoside Digestion Mix |
50 reactions |
|
14 |
New england biolabs |
P8109S |
Endoproteinase LysC |
20μg |
|
15 |
New england biolabs |
E8033S |
Anti-M13 pIII Monoclonal Antibody |
0.1 mL |
OneTaq® 2X Master Mix with Standard Buffer | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
OneTaq® 2X Master Mix with Standard Buffer
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
精选视频
产品信息
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Comparison of OneTaq Products to Other Commercially Available Polymerases
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
- 产品类别:
- OneTaq® DNA Polymerases Products,
- Taq DNA Polymerase Products,
- Master Mixes Products
- 应用:
- Fast PCR,
- Multiplex PCR,
- Specialty PCR,
- Routine PCR,
PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0482S -20 OneTaq® 2X Master Mix with Standard Buffer M0482SVIAL -20 2 x 1.25 ml 2 X
-
M0482L -20 OneTaq® 2X Master Mix with Standard Buffer M0482SVIAL -20 10 x 1.25 ml 2 X
-
- 特性和用法
1X 预混液组分
20 mM Tris-HCl
22 mM KCl
22 mM NH4Cl
1.8 mM MgCl2
5% Glycerol
0.05% Tween® 20
0.06% IGEPAL® CA-630
0.2 mM dNTPs
25 units/ml OneTaq® DNA Polymerase
pH 8.9 @ 25°C热失活
否
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
- 优势和特性
应用特性
- GC-rich PCR
- High Sensitivity PCR
- High Throughput PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic)
- AT-rich PCR
- 相关产品
相关产品
- Magnesium Chloride (MgCl2) Solution
- OneTaq® DNA Polymerase
- OneTaq® Hot Start DNA Polymerase
- OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
- OneTaq® Hot Start 2X Master Mix with Standard Buffer
- 注意事项
- OneTaq 2X Master Mix with Standard Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.
- OneTaq 2X Master Mix with Standard Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.
- Product specifications for individual components in the OneTaq 2X Master Mix with Standard Buffer are available separately.
- 参考文献
- Barnes,W.M. (1994). Proc. Natl Acad. Sci. USA. 91, 2216-2220.
- Saiki,R.K. et al (1985). Science. 230, 1350-1354.
- Powell,L.M. et. al. (1987). Cell. 50, 831-840.
- Sun,y., Hegamyer, G. and Colburn,N. (1993). Biotechniques. 15, 372-374.
- Sarker, G., Kapelner, S. and Summer, S.S. (1990). Nuclear Acids Res.. 18, 7465.
- Magda Matoušková, Pavel Veselý, Petr Daniel, Giada Mattiuzzo, Ralph Hector, Linda Scobie, Yasuhiro Takeuchi and Jiří Hejnar (2013). The Role of DNA Methylation in Expression and Transmission of Procine Endogenous Retrovirus. Journal of Virology. JVI.03262-12,
操作说明、说明书 & 用法
- 操作说明
- Protocol for OneTaq® 2X Master Mix with Standard Buffer (M0482)
- 使用指南
- Activity of Restriction Enzymes in PCR Buffers
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
- 应用实例
- Robust Colony PCR from Multiple E. coli Strains using OneTaq® Quick-Load® Master Mixes
工具 & 资源
- 选择指南
- DNA Polymerase Selection Chart
- Thermophilic DNA Polymerases
- Web 工具
- Tm Calculator
FAQs & 问题解决指南
- FAQs
- How should I set up a PCR using the OneTaq® Master Mixes?
- Can I use my regular Taq-based cycling conditions for OneTaq® DNA Polymerase based products?
- Can I use OneTaq® DNA Polymerase in “hot start” PCR to help with specificity or primer dimer problems?
- What are the stability and storage requirements of the OneTaq® Master Mixes?
- Can OneTaq® DNA Polymerase be used in colony PCR?
- What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
- How long a product can be made by OneTaq® DNA Polymerase?
- Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
- What is the fidelity of OneTaq® DNA Polymerase?
- Where can I find help troubleshooting my PCR?
- How should I determine an appropriate annealing temperature for my reaction?
- How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?
- 问题解决指南
- PCR Troubleshooting Guide
- 实验技巧
- Use OneTaq 2X Master Mix with Standard Buffer to amplify routine targets with up to 65% GC content.
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz更多引用文献
- Stöhr AC, López-Bueno A, Blahak S, Caeiro MF, Rosa GM, Alves de Matos AP, Martel A, Alejo A, Marschang RE (2015) Phylogeny and differentiation of reptilian and amphibian ranaviruses detected in europe PLoS One; 10(2), e0118633. PubMedID: 25706285, DOI: 10.1371/journal.pone.0118633
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- M0482S_L_v1
- M0482S_L_v2
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- M0482S_L_v1_0141612
- M0482S_L_v1_0151706
- M0482S_L_v1_0161712
- M0482L_v1_10008731
- M0482L_v1_10015061
- M0482S_v1_10014196
- M0482L_v1_10019978
- M0482S_v1_10026511
- M0482S_v1_10029430
- M0482L_v1_10026510
- M0482S_v1_10032470
- M0482L_v1_10036682
- M0482L_v1_10036899
- M0482L_v1_10039310
- M0482S_v1_10036683
- M0482L_v1_10042494
- M0482L_v1_10047023
- M0482S_v1_10047024
- M0482L_v1_10048754
- M0482S_v1_10050684
- M0482S_v1_10051130
- M0482L_v1_10051129
- M0482L_v1_10057774
- M0482S_v1_10057771
- M0482L_v1_10061437
- M0482L_v1_10062520
- M0482L_v2_10068553
- M0482S_v2_10068784
- M0482L_v2_10068785
- M0482L_v2_10071663
- M0482L_v2_10076981
- M0482L_v2_10080289
- M0482S_v2_10082678
- M0482L_v2_10082885
- M0482L_v2_10088143
- M0482S_v2_10088142
- M0482S_v2_10093280
- M0482L_v2_10093281
- M0482S_v2_10100280
- M0482L_v2_10100279
- M0482S_v2_10100564
- M0482L_v2_10104365
- M0482L_v2_10106904
- M0482L_v2_10109424
- M0482S_v2_10108349
- M0482L_v2_10109701
- M0482S_v2_10111048
- M0482L_v2_10115540
- M0482L_v2_10121320
- M0482S_v2_10122328
- M0482L_v2_10125363
- M0482S_v2_10127607
- M0482L_v2_10129101
- M0482L_v2_10130651
- M0482L_v2_10132264
- M0482S_v2_10135938
- M0482L_v2_10137223
- M0482S_v2_10141227
- M0482L_v2_10146099
- M0482S_v2_10146682
- M0482L_v2_10148706
- M0482S_v2_10151824
- M0482L_v2_10151437
- M0482L_v2_10159689
- M0482S_v2_10159711
- M0482S_v2_10163748
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
OneTaq® 2X Master Mix with Standard Buffer
-