单位定义

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.

反应条件

1X NEBuffer™ 2.1
Incubate at 37°C

1X NEBuffer™ 2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
100 µg/ml BSA
(pH 7.9 @ 25°C)

在不同缓冲液中的活性

NEBuffer™ 1.1: 100%
NEBuffer™ 2.1: 100%
NEBuffer™ 3.1: 10%

稀释兼容性

• 稀释液 C

贮存溶液

10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C

热失活

65°C for 20 min

甲基化敏感性

dam 甲基化: Not Sensitive
dcm 甲基化: Not Sensitive
CpG甲基化: Blocked by Some Combinations of Overlapping

相关产品

• NheI-HF®
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)
• Nuclease-free Water

单独销售的组分

• Gel Loading Dye, Purple (6X)
• NEBuffer 2.1

1. Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or XbaI.
2. Inhibited by salt concentrations > 100 mM.
3. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
4. Blocked by some combinations of overlapping CpG methylation.

1. Optimizing Restriction Endonuclease Reactions
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
• Activity of Restriction Enzymes in PCR Buffers
• Cleavage Close to the End of DNA Fragments
• Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
• Double Digests
• Effects of CpG Methylation on Restriction Enzyme Cleavage
• Heat Inactivation
• NEBuffer Activity/Performance Chart with Restriction Enzymes
• Optimizing Restriction Endonuclease Reactions
• Restriction Endonucleases – Survival in a Reaction
• Restriction Enzyme Diluent Buffer Compatibility
• Restriction Enzyme Tips
• Single Letter Codes
• Site Preferences
• Star Activity
• Traditional Cloning Quick Guide

• Alphabetized List of Recognition Sequences
• Cleavage of Supercoiled DNA
• Compatible Cohesive Ends and Generation of New Restriction Sites
• Dam-Dcm and CpG Methylation
• Frequencies of Restriction Sites
• Isoelectric Points (pI) for Restriction Enzymes
• Isoschizomers
• NEB Diluent and Buffer Table
• Recleavable Filled-in 5′ Overhangs
• Time-Saver™ Qualified Enzymes
• Why Choose Recombinant Enzymes?

• Competitor Cross-Reference Tool
• DNA Sequences and Maps Tool

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. Is NheI affected by methylation?
2. Are more units of NheI required to cut supercoiled DNA than lambda DNA?
3. Is NheI inhibited by salt?
4. Is NheI active at 25°C?
5. Does NheI produce commonly used compatible ends?
6. Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
7. Why is my Restriction Enzyme not cutting DNA?
8. Why do I see a DNA smear on an agarose gel after a restriction digest?
9. Why do I see additional DNA bands on my gel after a restriction digest?
10. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
11. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?

• Restriction Enzyme Troubleshooting Guide

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

• R0131M_v1
• R0131S_L_v1

CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

• R0131M_v1_0281306
• R0131M_v1_0291312
• R0131S_L_v1_0281301
• R0131S_L_v1_0291306
• R0131S_L_v1_0291312
• R0131M_v1_0271301
• R0131M_v1_0291405
• R0131S_L_v1_0271301
• R0131S_L_v1_0291405
• R0131M_v1_0291410
• R0131S_L_v1_0291410
• R0131S_L_v1_0291504
• R0131M_v1_0291504
• R0131S_L_v1_0291509
• R0131S_L_v1_0291604
• R0131M_v1_0291509
• R0131M_v1_0291605
• R0131S_L_v1_0291608
• R0131S_L_v1_0291611
• R0131S_L_v1_0291701
• R0131M_v1_0291705
• R0131S_L_v1_0291704
• R0131M_v1_0291711
• R0131S_L_v1_0291711
• R0131M_v1_0291701
• R0131M_v1_10011628
• R0131L_v1_10008860
• R0131S_v1_10015942
• R0131S_v1_10020681
• R0131S_v1_10027617
• R0131M_v1_10032186
• R0131S_v1_10035482
• R0131L_v1_10027620
• R0131L_v1_10042389
• R0131S_v1_10042391
• R0131M_v1_10053501
• R0131S_v1_10057181
• R0131L_v1_10063024
• R0131S_v1_10063025
• R0131M_v1_10071487
• R0131S_v1_10069390
• R0131S_v1_10078814
• R0131S_v1_10085530
• R0131S_v1_10091136
• R0131L_v1_10088050
• R0131M_v1_10088048
• R0131S_v1_10091579

以下 SDS 文件可以帮助您安全地使用该产品

• NheI