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OneTaq® Hot Start DNA Polymerase | NEB酶试剂 New England Biolabs

发表于2024年12月27日由Amresco中国官网

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

OneTaq^® Hot Start DNA Polymerase

The ONE polymerase for your endpoint PCR needs

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.

Unique hot start formulation eliminates non-specific amplification and does not require a separate activation step
Robust yield with minimal optimization
Ideal for routine, AT- or GC-rich templates
Room temperature reaction setup

精选视频

OneTaq® Hot Start DNA Polymerase | NEB酶试剂 New England Biolabs

How to Amplify GC-rich DNA

观看其他视频


货号	浓度	规格	目录价	北京	上海	广州	成都	苏州
M0481S	5,000 units/ml	200 units	¥889.00	有	有	无	无	无
M0481L	5,000 units/ml	1,000 units	¥3,559.00	有	无	无	无	无
M0481X	5,000 units/ml	5,000 units	¥14,209.00	无	无	无	无	无
如何订购
Please enter a quantity for at least one size

OneTaq® Hot Start DNA Polymerase | NEB酶试剂 New England Biolabs Want to try this product? Request a sample

产品信息

OneTaq^® Hot Start DNA Polymerase is an optimized blend of Taq and Deep Vent^® DNA polymerases combined with an aptamer-based inhibitor. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1) and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45°C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols.

OneTaq Hot Start DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons (i.e. ≥ 75% GC), the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent^® DNA Polymerase gene from Pyrococcus species GB-D.

产品类别:: OneTaq® DNA Polymerases Products,; Taq DNA Polymerase Products

应用:: Hot Start PCR,; Multiplex PCR,; Specialty PCR,; Routine PCR,

产品组分信息

本产品提供以下试剂或组分：

NEB #

名称

组分货号

储存温度

数量

浓度

M0481S

-20

OneTaq^® Hot Start DNA Polymerase	M0481SVIAL	-20	1 x 0.04 ml	5,000 units/ml
OneTaq^® Standard Reaction Buffer Pack	B9022SVIAL	-20	1 x 2 ml	5 X
OneTaq^® GC Reaction Buffer Pack	B9023SVIAL	-20	1 x 2 ml	5 X
OneTaq^® High GC Enhancer	B9026AVIAL	-20	1 x 2 ml	不适用

M0481L

-20

OneTaq^® Hot Start DNA Polymerase	M0481LVIAL	-20	1 x 0.2 ml	5,000 units/ml
OneTaq^® Standard Reaction Buffer Pack	B9022SVIAL	-20	5 x 2 ml	5 X
OneTaq^® GC Reaction Buffer Pack	B9023SVIAL	-20	3 x 2 ml	5 X
OneTaq^® High GC Enhancer	B9026AVIAL	-20	3 x 2 ml	不适用

M0481X

-20

OneTaq^® Hot Start DNA Polymerase	M0481LVIAL	-20	5 x 0.2 ml	5,000 units/ml
OneTaq^® Standard Reaction Buffer Pack	B9022SVIAL	-20	25 x 2 ml	5 X
OneTaq^® GC Reaction Buffer Pack	B9023SVIAL	-20	15 x 2 ml	5 X
OneTaq^® High GC Enhancer	B9026AVIAL	-20	15 x 2 ml	不适用

特性和用法

单位定义

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

反应条件

1X OneTaq^® Standard Reaction Buffer Pack

1X OneTaq^® Standard Reaction Buffer Pack
20 mM Tris-HCl
22 mM NH₄Cl
22 mM KCl
1.8 mM MgCl₂
0.06% IGEPAL® CA-630
0.05% Tween® 20
(pH 8.9 @ 25°C)

使用浓度

1.25 units/50μl reaction

贮存溶液

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

热失活

否

5′ – 3′ 核酸外切酶

Yes

3′ – 5′ 核酸外切酶

Yes

链置换

No

单位活性检测条件

1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
- High Sensitivity PCR
- High Throughput PCR
- Routine PCR
- GC-rich PCR
- AT-rich PCR
- Colony PCR
- Long PCR (up to ~6 kb genomic
相关产品
相关产品
- Deoxynucleotide (dNTP) Solution Set
- Deoxynucleotide (dNTP) Solution Mix
- Magnesium Chloride (MgCl₂) Solution
- Magnesium Sulfate (MgSO₄) Solution
- OneTaq® DNA Polymerase
单独销售的组分
- OneTaq® Standard Reaction Buffer Pack
- OneTaq® GC Reaction Buffer Pack
注意事项
1. The OneTaq High GC Enhancer should not be used alone. It should be added only to the OneTaq GC Reaction Buffer and will typically enhance yields when other conditions have failed.
2. Product specifications for individual components in the OneTaq Hot Start DNA Polymerase mix are available separately.
参考文献
1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki R.K. et al. (1985). Science. 230, 1530-1554.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.

操作说明、说明书 & 用法

操作说明
1. Protocol for OneTaq Hot Start DNA Polymerase (M0481)
使用指南
- Activity of Restriction Enzymes in PCR Buffers
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
应用实例
- Robust Colony PCR from Multiple E. coli Strains using OneTaq^® Quick-Load^® Master Mixes

工具 & 资源

选择指南
- DNA Polymerase Selection Chart
- Thermophilic DNA Polymerases
Web 工具
- Tm Calculator

FAQs & 问题解决指南

FAQs
1. How should I set up a PCR using OneTaq® Hot Start DNA Polymerase?
2. Can I use my regular Taq-based cycling conditions for OneTaq® DNA Polymerase based products?
3. Which buffer should I use?
4. How do I activate OneTaq® Hot Start Polymerase?
5. Is OneTaq® Hot Start DNA Polymerase active in other Taq reaction buffers?
6. When should I add the High GC Enhancer?
7. Can OneTaq® DNA Polymerase be used in colony PCR?
8. What type of DNA ends result from a primer extension reaction or a PCR using OneTaq® DNA Polymerase?
9. How long a product can be made by OneTaq® DNA Polymerase?
10. Can OneTaq® DNA Polymerase be used with uracil-containing primers or bisulfite-treated DNA?
11. What is the fidelity of OneTaq® DNA Polymerase?
12. Which buffer should I use if I want to control the level of magnesium in the reaction?
13. Where can I find help troubleshooting my PCR?
14. How should I determine an appropriate annealing temperature for my reaction?
15. How is OneTaq DNA Polymerase different from LongAmp™ Taq DNA Polymerase?
问题解决指南
- PCR Troubleshooting Guide
实验技巧

Did you know most OneTaq Hot Start reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

引用 & 技术文献

引用文献

产品引用文献查找工具

Powered by Bioz See more details on Bioz
专题文章
- Using aptamers to control enzyme activities: Hot Start Taq and beyond

质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
产品说明与变更通知
产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- M0481S_L_X_v1
- M0481S_L_X_v2
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- M0481S_L_X_v1_0101512
- M0481S_L_X_v1_0101606
- M0481S_L_X_v1_0101612
- M0481S_L_X_v1_0101706
- M0481S_L_X_v1_0101712
- M0481L_v1_10012698
- M0481S_v1_10012672
- M0481X_v1_10014190
- M0481X_v1_10018226
- M0481L_v1_10019278
- M0481X_v1_10019277
- M0481G_v0_10010785
- M0481X_v1_10023116
- M0481L_v1_10027986
- M0481X_v1_10027987
- M0481X_v1_10028225
- M0481S_v1_10030724
- M0481X_v1_10032797
- M0481X_v1_10034640
- M0481L_v1_10034534
- M0481X_v1_10038373
- M0481G_v0_10040424
- M0481L_v1_10035625
- M0481X_v1_10039159
- M0481X_v1_10042309
- M0481L_v1_10042310
- M0481X_v1_10042687
- M0481X_v1_10047068
- M0481L_v1_10048422
- M0481L_v1_10048747
- M0481X_v1_10048735
- M0481S_v1_10051085
- M0481L_v1_10054104
- M0481X_v1_10054103
- M0481S_v1_10054098
- M0481X_v1_10057356
- M0481S_v1_10057355
- M0481S_v1_10061082
- M0481L_v1_10060135
- M0481X_v1_10060860
- M0481G_v0_10060005
- M0481L_v1_10062271
- M0481L_v1_10063190
- M0481X_v1_10062270
- M0481L_v1_10064519
- M0481X_v1_10063898
- M0481L_v2_10065923
- M0481X_v2_10065924
- M0481X_v2_10068534
- M0481L_v2_10070052
- M0481X_v2_10070051
- M0481X_v2_10071139
- M0481S_v2_10068671
- M0481L_v2_10072336
- M0481X_v2_10073013
- M0481L_v2_10074869
- M0481X_v2_10074870
- M0481X_v2_10079006
- M0481L_v2_10082438
- M0481X_v2_10088678
- M0481L_v2_10092409
- M0481X_v2_10096732
- M0481X_v2_10097058
- M0481L_v2_10096733
- M0481S_v2_10095635
- M0481X_v2_10098356
- M0481L_v2_10101200
- M0481X_v2_10101201
- M0481G_v0_10103258
- M0481L_v2_10105512
- M0481X_v2_10105513
- M0481L_v2_10115544
- M0481X_v2_10114378
- M0481G_v0_10119920
- M0481S_v2_10118631
- M0481G_v0_10120891
- M0481L_v2_10117179
- M0481X_v2_10117180
- M0481L_v2_10120993
- M0481X_v2_10120994
- M0481G_v0_10127405
- M0481L_v2_10129099
- M0481X_v2_10129100
- M0481L_v2_10132263
- M0481X_v2_10134799
- M0481L_v2_10138006
- M0481X_v2_10138005
- M0481G_v1_10142489
- M0481L_v2_10139838
- M0481X_v2_10139839
- M0481L_v2_10144877
- M0481S_v2_10141008
- M0481X_v2_10144876
- M0481L_v2_10148034
- M0481G_v1_10149467
- M0481G_v1_10157100
- M0481G_v1_10158544
- M0481S_v2_10160179
- M0481L_v2_10156027
- M0481X_v2_10148700
SDS
以下 SDS 文件可以帮助您安全地使用该产品
- OneTaq^® Hot Start DNA Polymerase
- OneTaq^® High GC Enhancer

鹅骨髓单个核细胞分离液试剂盒,货号：P5100

发表于2024年12月27日由Amresco中国官网

鹅骨髓单个核细胞分离液试剂盒,货号：P5100

市场价：

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价格：

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品牌：

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产品详情

说明书下载

参考文献

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产品咨询

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德国IKA|艾卡加热板C-MAG HP 7/C-MAG HP 4

发表于2024年12月27日由Amresco中国官网

德国IKA|艾卡加热板C-MAG HP 7/C-MAG HP 4

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固定安全温度：温度超过550℃即停止加热
热警显示：加热时指示灯闪烁，警告用户加热盘高温，不可触摸
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德国IKA|艾卡加热板C-MAG HP 7/C-MAG HP 4

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最大容量：10 l

Brand普兰德组装式自动回零滴定管 10ml （23819）

发表于2024年12月27日由Amresco中国官网

Brand普兰德组装式自动回零滴定管 10ml （23819）

商品品牌： Brand普兰德

商品编号：23819

商品价格：请与我们联系

Brand普兰德组装式自动回零滴定管 10ml （23819）-Brand普兰德-23819

容量:10ml
颜色:透明

级别:A级(带计量证书)
滴定方式:自动回零式

品牌属性:进口

实验室耗材|||分析计量|||滴定管|||Brand普兰德组装式自动回零滴定管10ml（23819）

滴定管

Brand普兰德组装式自动回零滴定管 10ml （23819）

1、组装式自动回零滴定系统理念！

2、快速拆卸，易于清理，维护简单 – 所有零部件都可拆卸更换！

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4、使用DURAN® 玻璃, 可拆卸的PTFE滴定阀。

5、参照DIN EN ISO 385标准。（TD，EX）校准。

6、如有需要，可以另行提供个体检验证书，USP个体证书或DKD校准证书。

7、所有BLAUBRAND® 自动回零滴定管提供独立批号，并在原包装中附有该

产品批号的批次检验证书。

刻度与阅读


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关于普兰德

当今严格的科学研究和分析要求最高质量的实验室产品。 BRAND与世界各地的客户一起不断改进和调整我们的产品以应对各种不断变化的应用需求。

BRAND的目标是开发和提供能够支持您特殊需求，满足您在性能和舒适度上的要求的实验室设备。

我们的内部研发人员是BRAND品牌质量哲学的基础。最新的生产和计算机技术整合在质量管理和设计领域使我们为客户提供性能尽可能高的产品成为可能。

商品属性

容量:10ml
颜色:透明

级别:A级(带计量证书)
滴定方式:自动回零式

品牌属性:进口

商品属性
商品名称	Brand普兰德组装式自动回零滴定管 10ml （23819）-23819-Brand普兰德
型号	23819
类别	实验室耗材\|\|\|分析计量\|\|\|滴定管\|\|\|Brand普兰德组装式自动回零滴定管10ml（23819）
品牌	Brand普兰德
品牌简介	Brand普兰德
关键字	滴定管,滴定管,刻度,普兰,可拆卸,标记,证书

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OneTaq® Hot Start DNA Polymerase

精选视频

How to Amplify GC-rich DNA

产品来源

产品组分信息

单位定义

反应条件

使用浓度

贮存溶液

热失活

5′ – 3′ 核酸外切酶

3′ – 5′ 核酸外切酶

链置换

单位活性检测条件

应用特性

相关产品

单独销售的组分

产品引用文献查找工具

产品说明与变更通知

affbiotech 代理

Brand普兰德 组装式自动回零滴定管 10ml （23819）

Brand普兰德 组装式自动回零滴定管 10ml （23819）-Brand普兰德-23819

商品属性

OneTaq^® Hot Start DNA Polymerase

Brand普兰德组装式自动回零滴定管 10ml （23819）

Brand普兰德组装式自动回零滴定管 10ml （23819）-Brand普兰德-23819