标签归档：原液

封闭用正常山羊血清（原液）

发表于由

上海金畔生物科技有限公司提供封闭用正常山羊血清（原液） ，欢迎访问官网了解更多产品信息。

产品编号 C-0005
英文名称 Normal Goat Serum
中文名称 封闭用正常山羊血清（原液）
别    名 NORMAL GOAT SERUM (NGS); Goat Serum; blocking serum;   山羊血清; 山羊血清封闭液; 正常山羊血清; 正常血清; 动物非免疫血清（羊）;
封闭用正常山羊血清（原液）
Specific References  (40)     |     C-0005 has been referenced in 40 publications.
[IF=11.205] Yingchun Luo. et al. Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis. EBIOMEDICINE. 2022 Aug;82:104087  
PubMed:35797768

[IF=9.236] Wei, Yuchun. et al. [18F]AlF-NOTA-FAPI-04: FAP-targeting specificity, biodistribution, and PET/CT imaging of various cancers. Eur J Nucl Med Mol I. 2022 Mar;:1-13  Other ;  Other.  
PubMed:35262766

[IF=8.579] Zhang J et al. Dual inhibition of PFKFB3 and VEGF normalizes tumor vasculature, reduces lactate production, and improves chemotherapy in glioblastoma: insights from protein expression profiling and MRI. Theranostics . 2020 Jun 5;10(16):7245-7259.  IHC-P&IHF ;  
PubMed:32641990

[IF=7.717] Liu, Li. et al. OTUB2 Regulates YAP1/TAZ to Promotes the Progression of Esophageal Squamous Cell Carcinoma. BIOL PROCED ONLINE. 2022 Dec;24(1):1-14  
PubMed:35850645

[IF=6.217] Zhu H et al. ALKBH5 inhibited autophagy of epithelial ovarian cancer through miR-7 and BCL-2. J Exp Clin Cancer Res. 2019 Apr 15;38(1):163.  IHC ;  
PubMed:30987661

[IF=6.17] Xiaoping Liu. et al. BRG1 protects the heart from acute myocardial infarction by reducing oxidative damage through the activation of the NRF2/HO1 signaling pathway. Free Radical Bio Med. 2020 Nov;160:820  
PubMed:32950688

[IF=6.058] He Bing-Qiang. et al. Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos. NEURAL REGEN RES. 2023 Jan;:  IF ;  Gecko.  
PubMed:10.4103/1673-5374.366495

[IF=5.793] Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16  IHC ;  Mouse.  
PubMed:33070776

[IF=5.714] Tengyun Yang. et al. MicroRNA-146a-5p alleviates the pathogenesis of osteoarthritis by inhibiting SDF-1/CXCR4-induced chondrocyte autophagy. INT IMMUNOPHARMACOL. 2023 Apr;117:109938  
PubMed:36863142

[IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080  IF ;  Human.  
PubMed:32417913

[IF=4.996] Li, Meng. et al. Dynamic expression of Mage-D1 in rat dental germs and potential role in mineralization of ectomesenchymal stem cells. SCI REP-UK. 2022 Dec;12(1):1-13  
PubMed:36585447

[IF=4.799] Ranran Zhang. et al. The Role of SREC-Ⅰ in Innate Immunity to Aspergillus fumigatus Keratitis. Invest Ophth Vis Sci. 2021 Jul;62(9):12-12  IF ;  Mouse.  
PubMed:34236384

[IF=4.49] Gan M. et al. miR-222 is involved in the regulation of genistein on skeletal muscle fiber type. The Journal of Nutritional Biochemistry(2019).  ICF ;  
PubMed:DOI:10.1016/j.jnutbio.2019.108320

[IF=4.362] JunXia Gao. et al. GLT-1 Knockdown Inhibits Ceftriaxone-Mediated Improvements on Cognitive Deficits, and GLT-1 and xCT Expression and Activity in APP/PS1 AD Mice. Front Aging Neurosci. 2020; 12: 580772  
PubMed:33132901

[IF=3.461] Wang H et al. α-PD-L1 mAb enhances the abscopal effect of hypo-fractionated radiation by attenuating PD-L1 expression and inducing CD8+ T-cell infiltration.(2018) Immunotherapy  IHC-P ;  
PubMed:30511887

[IF=3.417] Shuchong Mei. et al. Expression and role of fibroblast activation protein α in acute myeloid leukemia. Oncol Rep. 2021 Feb;45(2):641-651  other ;  
PubMed:33416165

[IF=3.249] Xue C et al. The Down-Regulation of SUZ12 Accelerates the Migration and Invasion of Liver Cancer Cells via Activating ERK1/2 Pathway.J Cancer. 2019 Feb 23;10(6):1375-1384.  IHC-P ;  
PubMed:31031847

[IF=3.231] Qian Chen. et al. Transcriptomic Analysis Reveals mRNA and Alternative Splicing Events in Ovine Skeletal Muscle Satellite Cells during Proliferation and Differentiation. ANIMALS. 2023 Jan;13(6):1076  
PubMed:36978617

[IF=3.171] Jian-Zhu Bo. et al. D-serine reduces memory impairment and neuronal damage induced by chronic lead exposure. Neural Regen Res. 2021 May;16(5):836  
PubMed:33229717

[IF=3.038] Fan Zhang et al. Early Intervention of Gastrodin Improved Motor Learning in Diabetic Rats Through Ameliorating Vascular Dysfunction. Neurochem Res. 2020 Aug;45(8):1769-1780.  IF ;  Rat.  
PubMed:32415405

[IF=2.985] Anxiong Liu. et al. Mild hypothermia pretreatment extenuates liver ischemia-reperfusion injury through Rab7-mediated autophagosomes-lysosomes fusion. Biochem Bioph Res Co. 2021 Apr;550:15  IF ;  
PubMed:33677131

[IF=2.98] Fu, Zhenzhen. et al. Using whole exome sequencing to identify susceptibility genes associated with nonsyndromic cleft lip with or without cleft palate. MOL GENET GENOMICS. 2022 Nov;:1-12  
PubMed:36322204

[IF=2.795] Chao Ma. et al. CXCL1 stimulates decidual angiogenesis via the VEGF-A pathway during the first trimester of pregnancy. 2021 Mar 26  IHC ;  
PubMed:33770315

[IF=2.784] Ma C et al.Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos.Int J Mol Med. 2018 Jul;42(1):525-533.  ICF ;  
PubMed:29693133

[IF=2.682] Chen Zhiping. et al. Mitofusin-2 Restrains Hepatic Stellate Cells’ Proliferation via PI3K/Akt Signaling Pathway and Inhibits Liver Fibrosis in Rats. J Healthc Eng. 2022;2022:6731335  Other ;  Other.  
PubMed:35083025

[IF=2.564] Wang JJ et al. Seasonal expressions of androgen receptor, P450arom and estrogen receptors in the epididymis of the wild ground squirrel (Citellus dauricus Brandt). General and Comparative Endocrinology, 2019 270, 131–138.  IHC-P ;  
PubMed:doi:10.1016/j.ygcen.2018.10.017

[IF=2.445] Yuan Z et al. Seasonal expressions of oxytocin and oxytocin receptor in the epididymides in the wild ground squirrels (Citellus Dauricus Brandt). Gen Comp Endocrinol. 2020 Apr 1;289:113391.  IHC-P ;  
PubMed:31917151

[IF=2.311] Wang Q et al. Clinical and prognostic association of oncogene cadherin 11 in gastric cancer. Oncol Lett . 2020 Jun;19(6):4011-4023.  IHC ;  human.  
PubMed:32391104

[IF=2.276] Tan Ya. et al. Downregulated miR-204 Promotes Skeletal Muscle Regeneration. Biomed Res Int. 2020;2020:3183296  IHC ;  
PubMed:33282943

[IF=2.172] Yuchen Yao. et al. Seasonal expressions of VEGF and its receptors VEGFR1 and VEGFR2 in the prostate of the wild ground squirrels (Spermophilus dauricus). Eur J Histochem. 2021 Mar 24; 65(2): 3219  IHC ;  squirrel.  
PubMed:33764018

保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Application: Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭。可以封闭待检样本中与蛋白质非特异性结合的位点，另外还可以封闭样品中内源性的 Fc 片段结合位点，阻断抗体与样品中的 Fc 受体结合，从而降低背景，减少假阳性。在选择封闭血清时，要注意封闭用血清中不能含有目标蛋白，且与一抗来源不同，可用与二抗相同来源的封闭血清。正常山羊血清被广泛应用于组织和细胞染色中非特异性抗体结合的阻断，以及抗体的其他应用。血清可直接用于阻断，或作为阻断缓冲液的成分。

使用方法：

免疫组化：用5-10%正常山羊血清封闭（1:10-1:20倍稀释），室温孵育5-10分钟。清除血清，勿洗，滴加适当比例稀释的一抗或一抗工作液，37℃或室温孵育4℃过夜。

细胞涂片：用5-10%正常山羊血清封闭（1:10-1:20倍稀释），使用 PBS 稀释（ 0.01mol/L pH7.4），少量点滴：漠过细胞涂片即可.

ELISA：1:100倍稀释 使用 PBS 稀释（ 0.01mol/L pH7.4）37℃一小时。

封闭用正常兔血清（原液）

发表于由

上海金畔生物科技有限公司提供封闭用正常兔血清（原液） ，欢迎访问官网了解更多产品信息。

产品编号 C-0006
英文名称 Normal Rabbit Serum
中文名称 封闭用正常兔血清（原液）
别    名 Normal Rabbit serum. Whole serum is obtained from non-immunized (preimmune) healthy Rabbits. The serum is clarified through a 0.45micron low protein binding filter.  兔血清; 兔血清封闭液; 正常血清; 动物非免疫血清（兔）;
封闭用正常兔血清（原液）
Specific References  (3)     |     C-0006 has been referenced in 3 publications.
[IF=6.6] Lin Liu. et al. Giardia duodenalis and Its Secreted PPIB Trigger Inflammasome Activation and Pyroptosis in Macrophages through TLR4-Induced ROS Signaling and A20-Mediated NLRP3 Deubiquitination. Cells-Basel. 2021 Dec;10(12):3425  IF ;  Mouse.  
PubMed:34943932

[IF=3.448] Yong Y et al. Electroacupuncture pretreatment attenuates brain injury in a mouse model of cardiac arrest and cardiopulmonary resuscitation via the AKT/eNOS pathway. Life Sci. 2019 Aug 30;235:116821.  Other ;  
PubMed:31476306

[IF=2.425] Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1).  IHC-P ;  
PubMed:30652434

保存条件 Store at -20℃.
产品介绍 Rabbit Serum blocking reagent can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Application: Rabbit Serum blocking reagent is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.

封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭。可以封闭待检样本中与蛋白质非特异性结合的位点，另外还可以封闭样品中内源性的 Fc 片段结合位点，阻断抗体与样品中的 Fc 受体结合，从而降低背景，减少假阳性。在选择封闭血清时，要注意封闭用血清中不能含有目标蛋白，且与一抗来源不同，可用与二抗相同来源的封闭血清。正常兔血清被广泛应用于组织和细胞染色中非特异性抗体结合的阻断，以及抗体的其他应用。血清可直接用于阻断，或作为阻断缓冲液的成分。

使用方法

免疫组化： 用5-10%正常兔血清封闭（1:10-1:20倍稀释），室温孵育5-10分钟。清除血清，勿洗，滴加适当比例稀释的一抗或一抗工作液，37℃或室温孵育4℃过夜。

细胞涂片： 用5-10%正常兔血清封闭（1:10-1:20倍稀释），使用 PBS 稀释（ 0.01mol/L pH7.4），少量点滴：漠过细胞涂片即可.

封闭用正常小鼠血清（原液）

发表于由

上海金畔生物科技有限公司提供封闭用正常小鼠血清（原液） ，欢迎访问官网了解更多产品信息。

产品编号 C-0082
英文名称 Normal Mouse Serum
中文名称 封闭用正常小鼠血清（原液）
别    名   小鼠血清封闭液; 正常血清; 动物非免疫血清（小鼠）;
保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Normal mouse serum. Whole serum is obtained from non-immunized (preimmune) healthy mouses. The serum is clarified through a 0.45 micron low protein binding filter.

封闭血清适用于免疫组化或者免疫荧光实验中样本的封闭。可以封闭待检样本中与蛋白质非特异性结合的位点，另外还可以封闭样品中内源性的 Fc 片段结合位点，阻断抗体与样品中的 Fc 受体结合，从而降低背景，减少假阳性。在选择封闭血清时，要注意封闭用血清中不能含有目标蛋白，且与一抗来源不同，可用与二抗相同来源的封闭血清。正常小鼠血清可用于组织和细胞染色中非特异性抗体结合的阻断，以及抗体的其他应用。血清可直接用于阻断，或作为阻断缓冲液的成分。

使用方法
免疫组化： 用5-10%正常小鼠血清封闭（1:10-1:20倍稀释），室温孵育5-10分钟。清除血清，勿洗，滴加适当比例稀释的一抗或一抗工作液，37℃或室温孵育4℃过夜。
细胞涂片： 用5-10%正常小鼠血清封闭（1:10-1:20倍稀释），使用 PBS 稀释（ 0.01mol/L pH7.4），少量点滴：漠过细胞涂片即可.

封闭用兔血清（原液）

发表于由

封闭用兔血清（原液）

￥40.00

货号：BL937A

规格：10ml

品牌：Jinpan

产品简介：

本产品用于免疫标记实验中样本的封闭。封闭用血清可以封闭样本中于蛋白质非特异性结合的位点，以及内源性Fc片段结合位点，阻断抗体与样本中的Fc受体结合，从而降低背景，减少假阳性。在选择封闭血清时，需注意封闭用血清不能含有目的蛋白，不能与一抗来源相同，可用于二抗相同来源的血清。

本产品为封闭用兔血清原液，经无菌采集，三次0.1 um过滤后分装而成。

 

使用方法（仅供参考）：

1．使用时，用PBS缓冲液将封闭血清原液稀释10或20倍，配成10%或者5%的工作液，直接滴加在组织切片或者细胞凃片上，37℃孵育10-30分钟。

2．去除血清，勿清洗，直接滴加适当比例稀释的一抗工作液，37℃孵育1-2 h或4℃过夜。

 

注意事项：

1．使用前需4℃解冻并溶解均匀后方可使用，建议分装成小份后-20℃保存。

2．稀释后的血清不可保存太久，需尽快使用，以免细菌污染。建议现配现用。

3．本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。

4．为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

保存条件：

-20℃冷冻保存，一年有效。

货号 BL937A
规格 10ml
品牌 Jinpan
说明书下载 点击下载
  • 封闭用兔血清（原液）
  • 封闭用兔血清（原液）

封闭绵羊血清（原液）

发表于由

封闭绵羊血清（原液）

￥40.00

货号：BL938A

规格：10ml

品牌：Jinpan

产品简介：

本产品用于免疫标记实验中样本的封闭。封闭用血清可以封闭样本中于蛋白质非特异性结合的位点，以及内源性Fc片段结合位点，阻断抗体与样本中的Fc受体结合，从而降低背景，减少假阳性。在选择封闭血清时，需注意封闭用血清不能含有目的蛋白，不能与一抗来源相同，可用于二抗相同来源的血清。

本产品为封闭用绵羊血清原液，经无菌采集，三次0.1 um过滤后分装而成。

 

使用方法（仅供参考）：

1．使用时，用PBS缓冲液将封闭血清原液稀释10或20倍，配成10%或者5%的工作液，直接滴加在组织切片或者细胞凃片上，37℃孵育10-30分钟。

2．去除血清，勿清洗，直接滴加适当比例稀释的一抗工作液，37℃孵育1-2h或4℃过夜。

 

注意事项：

1．使用前需4℃解冻并溶解均匀后方可使用，建议分装成小份后-20℃保存。

2．稀释后的血清不可保存太久，需尽快使用，以免细菌污染。建议现配现用。

3．本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。

4．为了您的安全和健康，请穿实验服并戴一次性手套操作。

 

保存条件：

-20℃冷冻保存，一年有效。

货号 BL938A
规格 10ml
品牌 Jinpan
说明书下载 点击下载
  • 封闭绵羊血清（原液）