| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRO0353 | pSUPER.retro.puro | Oligoengine | RNAi载体 |
Plasmid Type: Mammalian Expression, RNAi
Size: 6349
Bacterial Resistance: Ampicillin
Selectable Marker: Puromycin
Viral/Non-Viral: Retroviral
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VNC0418 | pQCXIH | Clontech | 逆转录病毒载体 |
Promotor: CMV/MSV
Size: 7800
5′ Sequencing 1 Primer: QC
5′ Sequencing 1 Primer Sequence: 5’d[ACGCCATCCACGCTGTTTTGACCT]3′
Tag 1: IRES-Hygro
Selectable Marker: Hygromycin
Viral/Non-Viral: Retroviral
RNAi载体，pSilencer5.1-H1 Retro载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0581 | pSilencer5.1-H1 Retro | Ambion | RNAi载体 |
pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-H1 Retro uses the H1 promoter.
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VSC0467 | pTK-hyg | Clontech | 四环素调控系统 |
Description
pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the
selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable
cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an
enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived
plasmids upon cointegration into the host cell’s genome.
Use
pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains
a gene of interest using any standard transfection technique. Most mammalian cell lines
require 200 µg/ml of Hygromycin to select for stably transformed cells.
Location of Features
• HSV TK polyA signals: 1473–1478 & 1486–1491 （complementary）
• Hygr
（hygromycin resistance gene）: 1537–2574 （complementary）
• P
HSV TK （HSV TK promoter）: 2588–2835 （complementary）
• pUC origin ofreplication: 3152–3795
• Ampr
（ampicillin resistance gene; beta-lactamase）: 3943–4803
Propagation in E. coli
• Suitable host strains: DH™ and other general purpose strains.
• Selectable marker: plasmid confers resistance to ampicillin （50 µg/ml） on E. coli hosts.
• E. coli replication origin: pUC
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VSC0466 | pTRE2 hyg | Clontech | 四环素调控系统 |
Promotor: Tet-responsive
Size: 5300
Selectable Marker: Hygromycin
Constitutive: Inducible
Viral/Non-Viral: Nonviral