上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
PNGase F, Recombinant
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F, Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
- Recombinant enzyme; guaranteed endoglycosidase F1-, F2- or F3-free
- Leaves N-glycan core oligosaccharides intact and suitable for further analysis
- ≥ 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Stored in 50% glycerol
- Optimal activity and stability for up to 24 months
- Can be used under native or denaturing conditions
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Rapid PNGase F |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Rapid™ PNGase F
Deglycosylation in minutes for N-glycan analysis
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- ≥ 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
- Fast reaction setup
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Rapid™ PNGase F (non-reducing format) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Rapid™ PNGase F (non-reducing format)
Deglycosylation in minutes for intact antibody analysis
Developed for proteomic applications, Rapid PNGase F (non-reducing format) enables complete and rapid deglycosylation, while preserving disulfide bonds. This facilitates high throughput proteomics applications and methods for antibody characterization by mass spectrometry, such as intact mass analysis. Rapid PNGase F (non-reducing format) combines the advantages of Rapid PNGase F (fast processing time), with non-reducing conditions, preserving quaternary structure.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 15 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability for up to 12 months
- Fast reaction setup
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Rapid PNGase F Antibody Standard |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Rapid PNGase F Antibody Standard
Rapid PNGase F Antibody Standard is a murine anti-MBP monoclonal antibody, isotype IgG2a. This antibody standard can be used as a positive control for Rapid PNGase F.
- Contains typical IgG glycosylation in a conserved Asn (#295) of the Fc region
- Optimal stability for up to 24 months
