标签归档：set

Acyclonucleotide Set | NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

Acyclonucleotide Set

Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). 
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5). 

Acyclonucleotide Set | NEB酶试剂 New England Biolabs

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N0460S 不适用 0.5 µmol ¥849.00 有 有 有 无 无
如何订购
Please enter a quantity for at least one size

产品信息

When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ (see figure).

Acyclonucleotide Set | NEB酶试剂 New England Biolabs
(50 ng/µl single-stranded M13mp18 DNA, 0.05 µM [32P]-S1224S primer, 1X ThermoPol Buffer, 50 µM dNTP, varying acyGTP and 0.05 units/µl Therminator) were mixed, incubated at 94°C for 5 minutes and then thermal cycled for 25 cycles at 94°C (30 seconds); 55°C (30 seconds); 72°C (30 seconds). Reaction products were separated on a denaturing polyacrylamide gel (QuickPoint, NOVEX, Inc.) and analyzed by autoradiography. Numbers above each lane give the molar ratio of acyGTP to dNTP in each lane (i.e., 50, 17, 5,6 and 1.9 µM acyGTP, respectively). Numbers to the side of the gel indicate the length of the primer extension product added to the primer.
产品类别:
Nucleotide Solutions Products

应用:
DNA Sequencing,
PCR,
PCR

  • 试剂盒组成

    试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N0460S     -20    
        AcycloATP N0461AVIAL -20 1 x 0.5 µmol 不适用
        AcycloCTP N0462AVIAL -20 1 x 0.5 µmol 不适用
        AcycloGTP N0463AVIAL -20 1 x 0.5 µmol 不适用
        AcycloTTP N0464AVIAL -20 1 x 0.5 µmol 不适用

  • 特性和用法

  • 相关产品

    相关产品

    • Therminator™ DNA Polymerase

  • 注意事项
    1. Thermo Sequenase™ is a trademark of GE Healthcare.
    2. Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.

  • 参考文献
    1. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
    2. Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
    3. Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
    4. Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2553.
    5. Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res.. 30(2), 605-613.

FAQs & 问题解决指南

  • FAQs
    1. Are substrates terminated with 3′ acyclonucleosides resistant to 3′-5′ exonuclease activity?
    2. Which DNA polymerases incorporate acyclonucleotides?
    3. Can acyclonucleotides be used in Sanger sequencing?
    4. Can acyclonucleotides be used for sequencing-by-synthesis?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Acyclonucleotide Set | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

  • 出版物
    • Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234​, DOI: 10.3389/fmolb.2019.00028

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N0460S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N0460S_v0_10013356
    • N0460S_v1_10013356
    • N0460S_v1_10022005
    • N0460S_v1_10048656
    • N0460S_v1_10092480
    • N0460S_v1_10123991
    • N0460S_v1_10151064
    • N0460S_v1_10154923
    • N0460S_v1_10157552
    • N0460S_v1_10163039

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • AcycloATP
    • AcycloCTP
    • AcycloGTP
    • AcycloTTP

Acyclonucleotide Set |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

Acyclonucleotide Set

Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). 
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5). 

Acyclonucleotide Set |

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
N0460S 不适用 0.5 µmol ¥849.00 有 有 有 无 无
如何订购
Please enter a quantity for at least one size

产品信息

When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ (see figure).

Acyclonucleotide Set |
(50 ng/µl single-stranded M13mp18 DNA, 0.05 µM [32P]-S1224S primer, 1X ThermoPol Buffer, 50 µM dNTP, varying acyGTP and 0.05 units/µl Therminator) were mixed, incubated at 94°C for 5 minutes and then thermal cycled for 25 cycles at 94°C (30 seconds); 55°C (30 seconds); 72°C (30 seconds). Reaction products were separated on a denaturing polyacrylamide gel (QuickPoint, NOVEX, Inc.) and analyzed by autoradiography. Numbers above each lane give the molar ratio of acyGTP to dNTP in each lane (i.e., 50, 17, 5,6 and 1.9 µM acyGTP, respectively). Numbers to the side of the gel indicate the length of the primer extension product added to the primer.
产品类别:
Nucleotide Solutions Products

应用:
DNA Sequencing,
PCR,
PCR

  • 试剂盒组成

    试剂盒组成

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • N0460S     -20    
        AcycloATP N0461AVIAL -20 1 x 0.5 µmol 不适用
        AcycloCTP N0462AVIAL -20 1 x 0.5 µmol 不适用
        AcycloGTP N0463AVIAL -20 1 x 0.5 µmol 不适用
        AcycloTTP N0464AVIAL -20 1 x 0.5 µmol 不适用

  • 特性和用法

  • 相关产品

    相关产品

    • Therminator™ DNA Polymerase

  • 注意事项
    1. Thermo Sequenase™ is a trademark of GE Healthcare.
    2. Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.

  • 参考文献
    1. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
    2. Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
    3. Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
    4. Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2553.
    5. Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res.. 30(2), 605-613.

FAQs & 问题解决指南

  • FAQs
    1. Are substrates terminated with 3′ acyclonucleosides resistant to 3′-5′ exonuclease activity?
    2. Which DNA polymerases incorporate acyclonucleotides?
    3. Can acyclonucleotides be used in Sanger sequencing?
    4. Can acyclonucleotides be used for sequencing-by-synthesis?

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    Acyclonucleotide Set | Powered by Bioz See more details on Bioz

  • 出版物
    • Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234​, DOI: 10.3389/fmolb.2019.00028

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • N0460S_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • N0460S_v0_10013356
    • N0460S_v1_10013356
    • N0460S_v1_10022005
    • N0460S_v1_10048656
    • N0460S_v1_10092480
    • N0460S_v1_10123991
    • N0460S_v1_10151064
    • N0460S_v1_10154923
    • N0460S_v1_10157552
    • N0460S_v1_10163039

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • AcycloATP
    • AcycloCTP
    • AcycloGTP
    • AcycloTTP

Subangstrom冷冻电镜Set of Metal Grid Boxes 8 Assorted Colors

发表于由
Subangstrom冷冻电镜Set of Metal Grid Boxes 8 Assorted Colors

Subangstrom冷冻电镜Set of Metal Grid Boxes 8 Assorted Colors

Subangstrom 位于纽约布鲁克林，致力于开发新产品以服务于冷冻电镜市场，可提供载网存储系统及配件和样品处理工具等，产品在国际上广受赞誉。


Set of Metal Grid Boxes 8 Assorted Colors 金属载网盒

货号：MGB01

含16个防碎透明针型盖子，可高压灭菌

颜色：红、黄、绿、蓝、紫、铬、青和金

Subangstrom冷冻电镜Set of Metal Grid Boxes 8 Assorted Colors


金畔生物是Subangstrom代理商，提供冷冻电镜相关产品

更多产品信息，请联系上海金畔生物科技有限公司

MiTeGen Clipping Tool Set M-CEM-NS-CLT-Set

发表于由
MiTeGen Clipping Tool Set M-CEM-NS-CLT-Set

MiTeGen为广大客户提供：Nanosoft系列产品，Nanosoft的工具旨在使您的冷冻电镜工作更加高效和富有成效。其中部分工具专门适配FEI/Thermo Fisher Scientific Vitrobot使用。


Clipping Tool Set

M-CEM-NS-CLT-Set

Nanosoft Clipping Tools与赛默飞版本相同，装载有C-Clips，并且与赛默飞的clipping station兼容


Clipping Tool套装包括：

grid clipping tools，货号；M-CEM-NS-CLT01（4个）

MiTeGen Clipping Tool Set M-CEM-NS-CLT-Set

Nanosoft Grid Clipping Tools is loaded with c-clips and clips grids the same way as the Thermo Fisher version, and is compatible with the Thermo Fisher clipping station. The difference is that with the Nanosoft version, instead of ejecting the C-clip by pressing a long plastic handle like with the Thermo version, you press on the Nanosoft tool with a pair of tweezers.

The Nanosoft version has the advantage of a compact design so that when they are placed in LN2 in the clipping station to be cooled down, the entire body is deep enough inside the station that they are untouched by any frost from environmental moisture, even when left in the station at cryogenic temperature for long periods of time. This means you can pre-load as many of the Nanosoft tools with C-clips as you would like, cool them all down at the same time, and then clip all your grids at one time without with little consideration for ice contamination – no need to warm, re-load and re-cool clipping tools.

C-clip bottoming tool，货号；M-CEM-NS-CCBT01（1个）

MiTeGen Clipping Tool Set M-CEM-NS-CLT-Set

The Nanosoft C-clip Bottoming Tool provides an easier and more efficient way to “bottom” the C-clip at the tip of the barrel of a clipping tool prior to clipping. After pressing the c-clip into the barrel of the tool, instead of pressing the tip of the barrel against the tabletop or a pair of tweezers to push the c-clip to the tip of the barrel (by pressing the plunger), you place the barrel into the Bottoming Tool and press the plunger of the tool to bottom the c-clip.

The cylinder of the Bottoming Tool ensures that the barrel of the clipping tool is straight, so upon pressing of the plunger to push the c-clip to the tip you are much less likely to “mis-bottom” the c-clip. Often when pressing the tip against the table top or another flat surface, you are not able to hold the barrel exactly straight again the surface and you the c-clip can fly out of the tool and is lost. The Nanosoft Bottoming Tool provides an easy way to ensure you don’t mis-bottom c-clips anymore.


clipping tool rack，货号；M-CEM-NS-CCBT01（1个）

MiTeGen Clipping Tool Set M-CEM-NS-CLT-Set


更多产品信息，请联系上海金畔生物科技有限公司