聚乙二醇辛基苯基醚

上海金畔生物科技有限公司提供聚乙二醇辛基苯基醚 ,欢迎访问官网了解更多产品信息。

产品编号 C-0089
英文名称 Triton X-100
中文名称 聚乙二醇辛基苯基醚
别    名   辛基苯基聚氧乙烯醚; 聚乙二醇对异辛基苯基醚; 异辛基苯基聚氧乙烯醚; 乳化剂OP-10;
聚乙二醇辛基苯基醚
Specific References  (1)     |     C-0089 has been referenced in 1 publications.
[IF=6.706] Meng-Ling Zhang. et al. Nobiletin Inhibits Hypoxia-Induced Placental Damage via Modulating P53 Signaling Pathway. NUTRIENTS. 2022 Jan;14(11):2332  
PubMed:35684132

保存条件 Store at RT.
产品介绍 基本信息:
CAS:9002-93-1
分子式:(C2H4O)N·C14H22O
MDL:MFCD00128254
级别:CP
熔点:6℃
沸点:270℃
外观:透明至有点朦胧的液体
溶解性:能溶于水、甲苯、二甲苯和乙醇,不溶于石油醚。
用途/描述:Triton X-100(聚乙二醇辛基苯基醚) 是一种非离子型表面活性剂(或称去污剂)。分子量为646.86(C34H62O11)。它能溶解脂质,以增加抗体对细胞膜的通透性。免疫细胞化学中Triton X-100常用浓度为1%和0.3%,其中1%的Triton X-100常用于漂洗组织标本,0.3%的Triton X-100则常用于稀释血清,配制BSA等。但通常是先配制成30%的Triton X-100储备液,临用时稀释至所需浓度。

4′,6-联脒-2-苯基吲哚二盐酸盐

上海金畔生物科技有限公司提供4′,6-联脒-2-苯基吲哚二盐酸盐 ,欢迎访问官网了解更多产品信息。

产品编号 D-9106
英文名称 DAPI
中文名称 4′,6-联脒-2-苯基吲哚二盐酸盐
别    名 4′,6-Diamidino-2-phenylindole, dihydrochloride; DAPI dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; CAS:47165-04-8;   核酸染料;
4
Specific References  (33)     |     D-9106 has been referenced in 33 publications.
[IF=20.722] Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359  Mouse.  
PubMed:10.1016/j.nantod.2021.101359

[IF=10.317] Qi Sun. et al. Phototherapy and anti-GITR antibody-based therapy synergistically reinvigorate immunogenic cell death and reject established cancers. Biomaterials. 2021 Feb;269:120648  
PubMed:10.1016/j.biomaterials.2020.120648

[IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858  
PubMed:37039469

[IF=7.419] Honghua Yue. et al. Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model. BIOMED PHARMACOTHER. 2022 Oct;154:113573  Other ;  Other.  
PubMed:35987161

[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IF ;  Rat.  
PubMed:34474906

[IF=6.575] Qingqing Liu. et al. A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice Through Modulation of Gut Microbiota And Immune Imbalance. MOL NUTR FOOD RES. 2023 Jan;:2200428  
PubMed:36708241

[IF=6.518] Yingjun Liu. et al. A heparin–rosuvastatin-loaded P(LLA-CL) nanofiber-covered stent inhibits inflammatory smooth-muscle cell viability to reduce in-stent stenosis and thrombosis. J Nanobiotechnol. 2021 Dec;19(1):1-17  Other ;  
PubMed:33926468

[IF=6.208] Anqi Sun. et al. Serelaxin Alleviates Fibrosis in Thyroid-Associated Ophthalmopathy via the Notch Pathway. INT J MOL SCI. 2023 Jan;24(9):8356  
PubMed:37176063

[IF=6.17] Lu Qi. et al. The inhibition of colorectal cancer growth by the natural product macrocarpal I. Free Radical Bio Med. 2021 Jan;162:383  
PubMed:33137468

[IF=5.606] Luo Ping. et al. IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway. Rheumatology. 2020 Oct;59(10):3070-3080  
PubMed:32417913

[IF=5.344] Shenfei Zonget al. SERS-fluorescence-superresolution triple-mode nanoprobe based on surface enhanced Raman scattering and surface enhanced fluorescence. J Mater Chem B . 2020 Sep 23;8(36):8459-8466.  other ;  
PubMed:32812626

[IF=5.215] Ling-Zhu Shi. et al. N-Butanol Extract of Glycyrrhizae Radix et Rhizoma Inhibits Dengue Virus through Targeting Envelope Protein. PHARMACEUTICALS-BASE. 2023 Feb;16(2):263  
PubMed:10.3390/ph16020263

[IF=5.195] Chunyang Tian. et al. Identification of an effective fraction from Ampelopsis radix with anti-dengue virus activities in vitro and in vivo. J ETHNOPHARMACOL. 2023 Mar;:116339  
PubMed:36870463

[IF=5.058] Ping Hu. et al. The critical role of pancreatic stone protein/regenerating protein in sepsis-related multiorgan failure. FRONT MED-LAUSANNE. 2023; 10: 1172529  
PubMed:37215716

[IF=4.979] Wanlin Xu. et al. Biocompatible Polyurethane Conduit Grafted with Vascular Endothelial Growth Factor-loaded Hydrogel Repairs the Peripheral Nerve Defect in Rats. 2021 Dec 04  IF ;  Rat.  
PubMed:34863047

[IF=4.966] Ya‐Jing Yuan. et al. Shrm4 contributes to autophagy inhibition and neuroprotection following ischemic stroke by mediating GABAB receptor activation. Faseb J. 2020 Dec;34(12):15837-15848  IF ;  
PubMed:33079458

[IF=4.939] Qi Wang. et al. MR/NIRF Dual-Mode Imaging of αvβ3 Integrin-Overexpressing Tumors Using a Lipopeptide-Based Contrast Agent. Mol Pharmaceut. 2021;XXXX(XXX):XXX-XXX  IF ;  Mouse.  
PubMed:34677979

[IF=4.818] Zheng Mei. et al. Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p. J IMMUNOL RES. 2022;2022:4086710  
PubMed:35637792

[IF=4.799] Ranran Zhang. et al. The Role of SREC-Ⅰ in Innate Immunity to Aspergillus fumigatus Keratitis. Invest Ophth Vis Sci. 2021 Jul;62(9):12-12  IF ;  Mouse.  
PubMed:34236384

[IF=4.617] Wang, Yingli. et al. Ratiometric co-delivery of doxorubicin and paclitaxel prodrug by remote-loading liposomes for the treatment of triple-negative breast cancer. Drug Deliv Transl Re. 2022 Jan;:1-13  Other ;  Other.  
PubMed:35043372

[IF=4.545] Ke Maet al. Neuroprotective effect of Hongjing I granules on erectile dysfunction in a rat model of bilateral cavernous nerve injury. Biomed Pharmacother . 2020 Oct;130:110405.  other ;  
PubMed:32679461

[IF=4.145] Cuicui Hou. et al. Lysine demethylase 1B (Kdm1b) enhances somatic reprogramming through inducing pluripotent gene expression and promoting cell proliferation. EXP CELL RES. 2022 Nov;420:113339  Other ;  Other.  
PubMed:36075448

[IF=3.969] Li, Yuanfang. et al. Weighted gene co-expression network indicates that the DYNLL2 is an important regulator of chicken breast muscle development and is regulated by miR-148a-3p. BMC GENOMICS. Bmc Genomics. 2022 Dec;23(1):1-12  Other ;  Other.  
PubMed:35379193

[IF=3.946] Ziqi Chen. et al. Oxidative stress state inhibits exosome secretion of hPDLCs through a specific mechanism mediated by PRMT1. J PERIODONTAL RES. 2022 Sep;:  Other ;  Other.  
PubMed:36063421

[IF=3.906] Zhenzhen Wen. et al. RORβ suppresses the stemness of gastric cancer cells by downregulating the activity of the Wnt signaling pathway. Oncol Rep. 2021 Aug;46(2):1-11  Other ;  Human.  
PubMed:34278502

[IF=3.47] Zhaoxia Luo. et al. Effect of Electrical Stimulation of Cervical Sympathetic Ganglia on Intraocular Pressure Regulation According to Different Circadian Rhythms in Rats. Invest Ophth Vis Sci. 2020 Sep;61(11):40-40  IF ;  
PubMed:32976562

[IF=3.423] Jing Zhao. et al. Oxysophoridine protects against cerebral ischemia/reperfusion injury via inhibition of TLR4/p38MAPK‑mediated ferroptosis. MOL MED REP. 2023 Feb;27(2):1-11  
PubMed:36601753

[IF=3.378] Liu, Can. et al. Deficiency of Lactoferrin aggravates lipopolysaccharide-induced acute inflammation via recruitment macrophage in mice. BIOMETALS. 2022 Jun;:1-14  
PubMed:35650365

[IF=3.352] Yuanfang Li. et al. MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets. Poultry Sci. 2021 Nov;:101557  IF ;  Chicken.  
PubMed:34852967

[IF=3.169] Hak Myong Choe. et al. Altered fibrinogen level and fibrin clot structure in myostatin homozygous mutant pig. 2022 Mar 13  Other ;  Other.  
PubMed:35285059

保存条件 Store at -20℃, protect from light.
产品介绍 DAPI, or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

DAPI基本信息:
  级别/纯度:BR,98%
  CAS:28718-90-3
  分子式: C16H15N5·2HCl
  分子量: 350.25
  MDL: MFCD00012681

DAPI性质:
  1. 外观:黄色粉末
  2. 纯度:≥95% (HPLC)
  3. 产品描述:
  DAPI是一种可对DNA 染色的细胞核染色试剂,它在嵌入双链DNA后释放蓝色荧光。DAPI常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。尽管DAPI不能通过活细胞膜,但却能穿透扰乱的细胞膜而对核染色。DAPI具有很高的光漂白承受水平,能用来检测酵母线粒体DNA,叶绿体DNA,病毒DNA,microplasm DNA以及染色体DNA。DAPI-DNA复合物的激发和发射波长分别为360nm和460nm。
  4. 染色过程
  (1) 用1mL ddH2O将DAPI溶解,制得2.9mM的DAPI溶液(1mg DAPI/1mL H2O)。
  注:DAPI不能直接用PBS等缓冲溶液溶解,需要先用水将其溶解。
  (2) 取适量DAPI水溶液加到PBS中,制备成10~50µM的DAPI溶液。
  推荐以下PBS的配制方法:
  将8.0g NaCl、0.2g KCl、2.9g Na2HPO4·12H2O、0.2g KH2PO4 溶解于1L纯水中。
  (3) 将1/10培养基体积的DAPI溶液加入到细胞培养基中。也可以用1/10浓度的DAPI缓冲液代替培养基。
  (4) 在37℃培养细胞10~20分钟。
  (5) 用PBS或合适的缓冲液洗细胞两次。
  (6) 用带有360nm激发波长,460nm发射波长的滤光片的荧光显微镜观察细胞。

  储存条件:-20℃干燥避光保存,有效期一年。

  注意事项:
  DAPI对人体有一定刺激性,请注意适当防护。
  荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
  为减缓荧光淬灭可以使用抗荧光淬灭封片液。

四苯基卟啉

上海金畔生物科技有限公司提供四苯基卟啉 ,欢迎访问官网了解更多产品信息。

  • 产品信息
  • 常见问题
产品编号 D10043
英文名称 TPP
中文名称 四苯基卟啉
别    名 meso-Tetraphenylporphyrin; 5,10,15,20-Tetraphenylporphin; low chlorin 5,10,15,20-Tetraphenylporphyrin; Tetraphenylporphyrin;   间-四苯基卟吩; 内消旋-四苯基扑吩;
保存条件 Store at 2-8℃ stable for 4 years.Protect from light.
产品介绍 级别:AR,
CAS号:504-17-6
分子式:C4H4N2O2S
分子量:144.15
MDL:MFCD00006674
溶解性:溶于热水、乙醇、乙醚、稀碱溶液和稀盐酸,微溶于冷水(分解),水中溶解度: <6 g> 纯度:97%
外观/性状:类白色至黄色粉末。
敏感性:对光和空气敏感。
用途:用于定量检测脂多糖,卡拉胶和唾液酸类;用于检测脂质过氧化氢和脂质过氧化。
介绍:有恶臭。对空气敏感。半数致死量(小鼠,腹腔)600mg/kg。

1、抗体溶解方法
2、抗体修复方式
3、常用试剂的配制
4、免疫组化操作步骤
5、免疫组化问题解答
6、Western Blotting 操作步骤
7、Western Blotting 问题解答
8、关于肽链的设计
9、多肽的溶解与保存
10、酶标抗体效价测定程序