上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Ph.D.-7 Phage Display Peptide Library Kit v2
This product is a direct replacement for NEB #E8100S, Ph.D.™-7 Phage Display Peptide Library Kit
The Ph.D.-7™ Phage Display Peptide Library Kit v2 contains the Ph.D.-7 Phage Display Peptide Library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic Beads for a panning control experiment, and enough -96gIII sequencing primer for >50 sequencing reactions. The Ph.D.-7 Phage Display Peptide Library is a combinatorial library of random 7-mer peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The library consists of ~109 electroporated (i.e., unique) sequences.
- Ready to use complex phage library (~109 clones)
- Monoclonal antibody and Protein G Magnetic Beads included for new control panning experiment
- Panning against DYKDDDDK Mouse monoclonal antibody yields more reliable epitope results than former target protein (streptavidin)
- Does not require helper phage for amplification
- Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube
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Bulk packaging may also be available and requested for large recurring orders.
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Ph.D.™ Peptide Display Cloning System |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Ph.D.™ Peptide Display Cloning System
The Ph.D.™ Peptide Display Cloning System contains M13KE vector, the parent vector of NEB’s Ph.D. libraries. The included M13 extension primer is used to make DNA duplex out of a user provided synthetic oligo, with or without random bases. The display system is best used to display small peptides, i.e. sequences of 50 amino acids or less.
- M13KE Vector is based on M13mp18 containing the entire M13 genome, with specific cloning sites at the 5’ gIII, encoding pIII coat protein
- Allows for user-designed custom peptide library construction
- May be transfected into F’ E. coli to obtain infectious phage
