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LIPOPARTICLE TECHNOLOGY

Application Notes

THE LIPOPARTICLE
Lipoparticles incorporate high concentrations of properly folded and oriented membrane proteins on their surface. GPCRs, ion channels, and other complex membrane proteins can be incorporated into Lipoparticles at concentrations of 50-200 pmol/mg, 10-100 fold greater than cells or membrane preparations. Because Lipoparticles are virus-like particles （VLPs） based on the natural retroviral budding process, they are uniform in diameter （~150 nm） and maintain the orientation of incorporated membrane proteins. Integral Molecular produces, purifies, and delivers custom Lipoparticles containing user specified membrane proteins for applications in antibody development and drug discovery.

MODIFIED LIPOPARTICLES
Integral Molecular offers biochemically modified Lipoparticles containing biotin tags and fluorophores to enable membrane proteins to be captured onto solid supports and visualized without disrupting the conformation of incorporated receptor targets. Modified Lipoparticles are suited for use in specialized antibody development applications including:

ADVANTAGES
Lipoparticles offer distinct advantages over traditional sources of membrane proteins such as whole cells or membrane preparations:

Incorporation of difficult membrane proteins
Lipoparticles can incorporate oligomeric and multispanning membrane proteins in their correct topology. Receptors that are toxic or poorly trafficked can also be incorporated into Lipoparticles.

High purity and high concentrations of membrane proteins
Lipoparticles provide a source of concentrated membrane proteins that are free of cytoplasmic proteins and misoriented proteins, improving assay sensitivity and the specificity of immune responses.

LIPOPARTICLE STRUCTURE
The Lipoparticle structure is based on membrane-enveloped retroviruses. Non-infectious, retroviral virus-like particles （VLPs） are produced when the viral protein ‘Gag’ buds through the host cell membrane. The Gag protein forms a core which becomes enwrapped by the cell membrane. Once the membrane ‘pinches’ off, newly formed Lipoparticles diffuse away from the cell, carrying target membrane proteins with it. Since the lipid membrane surrounding the Lipoparticle is derived directly from the host cell membrane, the incorporated receptors remain correctly oriented in a native lipid environment. Lipoparticles contain no viral genome or Envelope protein, and are replication incompetent and non-infectious, rendering them safe to manipulate in a standard laboratory environment.

LIPOPARTICLE QUALITY CONTROL
At Integral Molecular, Lipoparticles undergo rigorous quality control before they are shipped. As a result, the Lipoparticles you receive are highly purified, homogeneous, and stable for nths to years. Maintaining

• Immunization
• Phage and yeast display
• Screening for and characterizing MAbs
• Ligand binding assays
• Probing cells and microarrays
• Biosensor binding kinetics

Application versatility
Lipoparticles can be used with conventional formats such as microplates and beads, but their small size, exceptional purity, and stability also make them amenable to formats that are otherwise difficult for membrane proteins, such as microfluidics, microarrays, and biosensors.
 

Integral Molecular是一家研究驱动的生物技术公司，为未被开发的膜蛋白靶标（包括GPCR，离子通道，转运蛋白和病毒包膜）创造创新技术和治疗性抗体。整合膜蛋白包括关键的治疗靶，但由于其结构复杂性和不能在构象完整状态下从脂质膜中除去而难以研究。在Integral Molecular，我们开发创新技术和产品，以满足这些苛刻但重要的目标。

脂质体技术

病毒样颗粒上的高浓度膜蛋白，用于药物和抗体的发现。

为什么我需要Lipoparticles用于抗体免疫和筛查？

脂质颗粒是病毒样颗粒（VLP），其天然构象含有高浓度的特异性膜蛋白。脂质颗粒直接从细胞表面捕获构象完整的膜蛋白，使得这些复合蛋白能够作为可溶性蛋白进行操作，用于抗体免疫和筛选。

Lipoparticle

为什么Lipoparticles是有效的抗原？

• Lipoparticles在其天然细胞膜中显示正确折叠的膜蛋白。这导致引发和筛选构象抗体的能力。
• 与细胞相比，脂质颗粒含有10-100倍浓度的膜蛋白（~50-200pmol / mg），导致强烈的免疫应答和抗体筛选的更好成功。

Lipoparticle应用包括：

• 免疫接种
• 噬菌体/酵母展示
• 通过ELISA筛选抗体
• 生物传感器的动力学分析
• 放射性配体和荧光结合试验

定制脂质颗粒

定制脂质颗粒以包含任何选择的膜蛋白。使用质量控制指标评估每批脂质颗粒，以确保均一性，纯度和目标蛋白质完整性。数百种膜蛋白靶标已成功掺入脂质颗粒中。对于特定应用，可以用生物素或荧光修饰脂质颗粒。

ReadyReceptor Lipoparticles

预先验证的Lipoparticles含有优化的，高度表达的膜蛋白，可用于快速递送。ReadyReceptor Lipoparticles使用Custom Lipoparticle生产中使用的所有严格质量指标生成和验证。

蛋GPCR

     Ion Channel

      RTK

      1-TM

    4-TM

    Viral Protein

    Transporter

Sample Kits

Lipoparticle sample kits containing CXCR4, CCR5 or CD20 are available for overnight delivery to enable application optimization and small-scale testing.

样品套件

含有CXCR4，CCR5或CD20的Lipoparticle样品试剂盒可用于隔夜输送，以实现应用优化和小规模测试。

脂质颗粒可以用（B）生物素或（F）荧光修饰。

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。

2：产品种类齐全，经营超过700多个品牌，基本涵盖所有生物实验试剂耗材。

3：提供加急服务，货品一般1-2周到货。

4：富有竞争力的价格优势，绝大部分价格有优势。

5：多年积累良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等药企。

6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。

7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。