上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Template Switching RT Enzyme Mix
The Template Switching RT Enzyme Mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. The mix contains a unique RT and Murine RNase Inhibitor. Unlike competitor RT products, no additives (such as PEG or betaine) are required for optimal performance, simplifying reaction setup. In conjunction with a template switching oligo (TSO), cDNA is synthesized with a known sequence of choice attached to the 3′ end. The resulting cDNA can be amplified by PCR or serve as template for 5′ RACE (rapid amplification of cDNA ends) or 2nd strand cDNA synthesis.
- Prepare RNA-seq libraries from extremely low input: single cells/nuclei or 2 pg of total RNA.
- Perform 5′ RACE from as low as 10 ng total RNA.
- Low background for RNA-seq or 5′ RACE
- Enzyme mix contains Murine RNase Inhibitor, no additives necessary
- Compatible with various template switching oligos (TSOs), RT primers, and DNA polymerases for full length cDNA amplification
- Enjoy faster protocols as compared to alternative RNA-seq methods (eg. Smart-Seq®)
产品信息
- 产品类别:
- cDNA Synthesis & Reverse Transcriptases Products,
- PCR, qPCR & Amplification Technologies Products
- 应用:
- cDNA Synthesis,
- RNA Analysis
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0466S -20 Template Switching RT Enzyme Mix M0466SVIAL -20 1 x 0.02 ml 10 X Template Switching RT Buffer B0466SVIAL -20 1 x 0.5 ml 4 X
-
M0466L -20 Template Switching RT Enzyme Mix M0466LVIAL -20 1 x 0.1 ml 10 X Template Switching RT Buffer B0466SVIAL -20 1 x 0.5 ml 4 X
-
- 特性和用法
操作说明、说明书 & 用法
- 操作说明
- cDNA Synthesis and Amplification Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
- 2nd Strand cDNA Synthesis Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
- 5’ RACE Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
FAQs & 问题解决指南
- FAQs
- Do I need to add RNase Inhibitor to the RT reaction when using the Template Switching RT Enzyme Mix?
- Numerous publications describe the addition of various additives (e.g., betaine and PEG) to improve template switching. Can I add any of those to my reaction?
- Is the Template Switching RT Enzyme Mix compatible with RT primers with degenerate bases for cDNA synthesis and amplification, as described in the mcSCRB method [1]?
- What type of template switching oligos (TSOs) are compatible with the Template Switching RT Enzyme Mix?
- Can I use the Template Switching RT Enzyme Mix for regular RT reactions?
- What is the efficiency of template switching when using the Template Switching RT Enzyme Mix?
- Does the Template Switching RT Enzyme Mix work for prokaryotic RNA?
- Does the Template Switching RT Enzyme Mix work for fragmented RNA?
- What type of RT primers can be used with the Template Switching RT Enzyme Mix?
- Can I reduce the RT reaction time?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- M0466S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- M0466L_v1_10034964
- M0466S_v1_10034965
- M0466L_v1_10054948
- M0466L_v1_10057544
- M0466L_v1_10059883
- M0466S_v1_10060350
- M0466S_v1_10063915
- M0466L_v1_10062116
- M0466L_v1_10070692
- M0466L_v1_10073395
- M0466L_v1_10073795
- M0466L_v1_10077107
- M0466S_v1_10073396
- M0466S_v1_10083313
- M0466L_v1_10084018
- M0466S_v1_10086688
- M0466L_v1_10088966
- M0466S_v1_10094233
- M0466L_v1_10094234
- M0466S_v1_10102585
- M0466S_v1_10105164
- M0466L_v1_10110683
- M0466S_v1_10118243
- M0466L_v1_10120363
- M0466L_v1_10122825
- M0466S_v1_10131915
- M0466S_v1_10135006
- M0466L_v1_10140691
- M0466S_v1_10139835
- M0466L_v1_10152833
- M0466S_v1_10154959
- M0466L_v1_10155310
- M0466S_v1_10156585
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
Template Switching RT Enzyme Mix
-
Template Switching RT Buffer
-
Template Switching RT Enzyme Mix |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Template Switching RT Enzyme Mix
The Template Switching RT Enzyme Mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. The mix contains a unique RT and Murine RNase Inhibitor. Unlike competitor RT products, no additives (such as PEG or betaine) are required for optimal performance, simplifying reaction setup. In conjunction with a template switching oligo (TSO), cDNA is synthesized with a known sequence of choice attached to the 3′ end. The resulting cDNA can be amplified by PCR or serve as template for 5′ RACE (rapid amplification of cDNA ends) or 2nd strand cDNA synthesis.
- Prepare RNA-seq libraries from extremely low input: single cells/nuclei or 2 pg of total RNA.
- Perform 5′ RACE from as low as 10 ng total RNA.
- Low background for RNA-seq or 5′ RACE
- Enzyme mix contains Murine RNase Inhibitor, no additives necessary
- Compatible with various template switching oligos (TSOs), RT primers, and DNA polymerases for full length cDNA amplification
- Enjoy faster protocols as compared to alternative RNA-seq methods (eg. Smart-Seq®)
产品信息
- 产品类别:
- cDNA Synthesis & Reverse Transcriptases Products,
- PCR, qPCR & Amplification Technologies Products
- 应用:
- cDNA Synthesis,
- RNA Analysis
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0466S -20 Template Switching RT Enzyme Mix M0466SVIAL -20 1 x 0.02 ml 10 X Template Switching RT Buffer B0466SVIAL -20 1 x 0.5 ml 4 X
-
M0466L -20 Template Switching RT Enzyme Mix M0466LVIAL -20 1 x 0.1 ml 10 X Template Switching RT Buffer B0466SVIAL -20 1 x 0.5 ml 4 X
-
- 特性和用法
操作说明、说明书 & 用法
- 操作说明
- cDNA Synthesis and Amplification Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
- 2nd Strand cDNA Synthesis Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
- 5’ RACE Protocol using the Template Switching RT Enzyme Mix (NEB #M0466)
FAQs & 问题解决指南
- FAQs
- Do I need to add RNase Inhibitor to the RT reaction when using the Template Switching RT Enzyme Mix?
- Numerous publications describe the addition of various additives (e.g., betaine and PEG) to improve template switching. Can I add any of those to my reaction?
- Is the Template Switching RT Enzyme Mix compatible with RT primers with degenerate bases for cDNA synthesis and amplification, as described in the mcSCRB method [1]?
- What type of template switching oligos (TSOs) are compatible with the Template Switching RT Enzyme Mix?
- Can I use the Template Switching RT Enzyme Mix for regular RT reactions?
- What is the efficiency of template switching when using the Template Switching RT Enzyme Mix?
- Does the Template Switching RT Enzyme Mix work for prokaryotic RNA?
- Does the Template Switching RT Enzyme Mix work for fragmented RNA?
- What type of RT primers can be used with the Template Switching RT Enzyme Mix?
- Can I reduce the RT reaction time?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- M0466S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- M0466L_v1_10034964
- M0466S_v1_10034965
- M0466L_v1_10054948
- M0466L_v1_10057544
- M0466L_v1_10059883
- M0466S_v1_10060350
- M0466S_v1_10063915
- M0466L_v1_10062116
- M0466L_v1_10070692
- M0466L_v1_10073395
- M0466L_v1_10073795
- M0466L_v1_10077107
- M0466S_v1_10073396
- M0466S_v1_10083313
- M0466L_v1_10084018
- M0466S_v1_10086688
- M0466L_v1_10088966
- M0466S_v1_10094233
- M0466L_v1_10094234
- M0466S_v1_10102585
- M0466S_v1_10105164
- M0466L_v1_10110683
- M0466S_v1_10118243
- M0466L_v1_10120363
- M0466L_v1_10122825
- M0466S_v1_10131915
- M0466S_v1_10135006
- M0466L_v1_10140691
- M0466S_v1_10139835
- M0466L_v1_10152833
- M0466S_v1_10154959
- M0466L_v1_10155310
- M0466S_v1_10156585
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
Template Switching RT Enzyme Mix
-
Template Switching RT Buffer
-
Micropoint Silicone Template报价
世界*实验材料供应商 micropoint-tech上海金畔生物为其中国代理, micropoint-tech在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, micropoint-tech就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Micropoint Technologies是cGMP兼容的和ISO 13485认证的医疗设备公司。我们有一个试验性的生产设施,以满足我们临床试验的需求。我们是一家前瞻性技术公司,为客户提供微针解决方案。我们的服务范围从快速成型,产品开发和微针OEM制造,包括不锈钢,塑料,聚合物,空心,固体,定制等所有类型。我们的试验设施能够生产用于临床的临床批次的溶解微针贴片试验。
我们提供的产品
Micropoint Technologies开发并提供以下产品:
- Micropoint PatchTM
- MPatchTM Mini
- MPatchTM Template
- PLancetTM
- Hollow Microneedle Hub
- One-Step Blood Sampling Platform
MPatchTM微针模板用于制造聚合物微针贴片。微针模板是通过在不锈钢微针母模上铸造室温硫化(RTV)硅酮来形成的。诸如水凝胶,脱乙酰壳多糖,透明质酸和糖的聚合物材料可以浇铸在微针模板上,并进行离心过程以形成尖锐的聚合物微针。这些微针模板非常耐用,在-50到+200摄氏度的工作温度范围内可以重复使用。
在批量生产中,这些有机硅模板是通过液体硅橡胶(LSR)注塑工艺制造的。在交付之前,模板也可以通过自动压紧进行消毒。
由LSR注塑成型的MPatch模板
定制的RTV硅胶模板
部分产品报价如下:
货号 | 品名 | 规格 | 目录价/10pc | 品牌 |
ST-15×15-H475B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-15×15-H525B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-15×15-H550B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-15×15-H575B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-15×15-H600B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H200B100P400 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H250B100P400 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H150B100P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H250B100P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H300B100P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H350B150P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H400B150P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H450B150P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H500B150P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H450B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H475B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H500B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H525B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H550B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H575B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H600B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H625B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H650B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H675B200P500 | Silicone Template | 8 mm x 8 mm | 8500 | micropoint-tech |
ST-10×10-H700B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H725B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H750B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H775B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H800B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H825B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-10×10-H850B200P500 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-8×8-H750B200P680 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-8×8-H775B200P680 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-8×8-H800B200P680 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-8×8-H825B200P680 | Silicone Template | 8 mm x 8 mm | 10200 | micropoint-tech |
ST-6×6-H800B250P500 | Silicone Template | 10 mm x 10 mm | 10200 | micropoint-tech |
ST-6×6-H1000B250P1000 | Silicone Template | 10 mm x 10 mm | 12750 | micropoint-tech |
ST-6×6-H1000B250P1500 | Silicone Template | 10 mm x 10 mm | 12750 | micropoint-tech |
ST-15×15-H500B200P1500 | Silicone Template | 30 mm x 30 mm | 17000 | micropoint-tech |
ST-40×80-H600B200P500 | Silicone Template | 22 mm x 44 mm | 无现货 | micropoint-tech |
ST-21×36-H300B200P2000 | Silicone Template | 48 mm x 78 mm | 2550/pc | micropoint-tech |
ST-25×90-H300B200P500 | Silicone Template | 34 mm x 67 mm | 2550/pc | micropoint-tech |