Tth Argonaute (TtAgo)

Thermus thermophilus argonaute (TtAgo) is a programmable DNA-endonuclease which requires a short 5’-phosphorylated single-stranded DNA guide to target its activity to a specific corresponding sequence on a substrate. TtAgo introduces one break in the phosphodiester backbone of the complementary substrate sequence.

• Short 16–18 nucleotide 5′-phosphorylated ssDNA guides are cost effective and can be phosphorylated with NEB T4 Polynucleotide Kinase
• Guide/target sequence selection is not limited by the requirement of an adjacent sequence motif
• Highly active on ssDNA and most dsDNA substrates (generates a nick in dsDNA substrates), with mild activity on ssRNA substrates
• Recommended for in vitro applications
• Refer to GUIDElines for optimization of Tth Argonaute (TtAgo) reactions