碱性磷酸酶标记生物素

上海金畔生物科技有限公司提供碱性磷酸酶标记生物素 ,欢迎访问官网了解更多产品信息。

产品编号 bs-0311P-AP
英文名称 Biotin/AP
中文名称 碱性磷酸酶标记生物素
别    名   生物素化碱性磷酸酶;
保存条件 Store at -20℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

碱性橙 14(电泳级)

上海金畔生物科技有限公司提供碱性橙 14(电泳级) ,欢迎访问官网了解更多产品信息。

产品编号 D10298
英文名称 Basic Orange 14
中文名称 碱性橙 14(电泳级)
别    名 Acridine Orange hemi(zinc chloride) sal; 3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt; 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt); Basic Orange 14;   吖啶橙半氯化锌盐; 吖啶橙; 碱性橙14; 3,6-双(二甲基氨基)吖啶氯化锌盐酸盐; 碱性橙 14, 染料含量52%;碱性橙 14, 染料含; 吖啶橙 生物染色; 吖啶橙 半氯化锌盐; 吖啶橙,半盐; 吖啶橙, 染料含量 55-60%; 碱性橙14,3,6-双(二甲氨基)吖啶; 丫啶橙 半氯化锌盐; RNA聚合酶抑制剂;
保存条件 Store at RT.
产品介绍 基本信息:
CAS号:10127-02-3
分子式:C17H20ClN3 · HCl · 1/2ZnCl2
分子量:369.96
MDL:MFCD00081043
级别:电泳级
纯度:80%
外观/性状:橙色至棕色粉末
溶解性:溶于水(1 mg/ml)、乙醇,溶液为橙黄色带绿色荧光。
敏感性:易吸潮
产品简介:
碱性橙 14 (Basic Orange 14)溶于水、乙醇,溶液为橙黄色带绿色荧光。pH值为8·4~10·4则无荧光。能镶嵌于两个相邻的碱基对之间,使部分双螺旋DNA松开,该两个碱基对的距离拉大一倍。这样的DNA复制过程中,会使DNA链增加或缺失一个碱基,造成移码突变。
用途:用作荧光指示剂,肿瘤细胞及细菌、核酸染色剂及移码突变的诱变剂。 吖啶橙是一种荧光色素,吖啶橙激发滤光片波长488nm。阻断滤光片波长515nm。吖啶橙与细胞中DNA和RNA结合量存在差别,可发出不同颜色的荧光(即着色特异性),这是由于DNA是个高度聚合物,吸收荧光物质的位置较少,发绿色荧光,而RNA聚合度低,能和荧光物质结合的位置多,故发红色荧光。

碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白

上海金畔生物科技有限公司提供碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白 ,欢迎访问官网了解更多产品信息。

产品编号 bs-0220P
英文名称 Alkaline Phosphatase(≥5,500 U/mg)
中文名称 碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白
别    名 Alkaline Phosphatase from calf intestine; Phosphatase, Alkaline from bovine intestinal mucosa; Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); ALP-1; ALP; ALPP; ALPPL2; ALPPL; PALP; PLAP-1; PLAP;   
碱性磷酸酶 (进口分装 ≥5,500 U/mg)蛋白
Specific References  (1)     |     bs-0220P has been referenced in 1 publications.
[IF=3.864] Xie W  et al. A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.Analyst. 2018 Jun 11;143(12):2901-2907.  other ;  
PubMed:29808208

保存条件 Stored at 2-8℃. Do not freeze.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Alkaline phosphatase is highly purified from calf intestine.
Alkaline phosphatase (ALP) removes phosphate groups from the 5′ end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays.

SWISS:
N/A

Gene ID:
N/A

CAS:9001-78-9
分子量:dimer ~160 kDa
MDL:MFCD00131849
浓度:≥10 mg/mL
活性:≥5500 DEA单位/mg蛋白质
单位定义:一个DEA单位将在37°C的pH 9.8下每分钟水解1μmol 4-硝基苯磷酸酯(一个甘氨酸单位相当于~3个DEA单位)
性状:溶液(含5 mM Tris、5 mM MgCl2和0.1 mM ZnCl2的50%甘油溶液,pH 7.0)
用途:碱性磷酸酶可以用来去磷酸酪蛋白和其他蛋白质。碱性磷酸酶也可以用来去磷酸化DNA或RNA的5′端,以防止自连接。用碱性磷酸酶去磷酸化后,DNA或RNA也可以用放射性标记的磷酸盐(通过T4多核苷酸激酶)标记。
推荐用于抗体和蛋白质结合的高比活性等级。

碱性磷酸酶(来源于牛肠粘膜,进口)

上海金畔生物科技有限公司提供碱性磷酸酶(来源于牛肠粘膜,进口) ,欢迎访问官网了解更多产品信息。

产品编号 D-9405
英文名称 Alkaline Phosphatase(≥5500 DEA u/mg) Protein
中文名称 碱性磷酸酶(来源于牛肠粘膜,进口)
别    名 Phosphatase, Alkaline from bovine intestinal mucosa; AP; ALP; Alkaline Phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1;   碱性磷酸酶;
保存条件 Store at 2-8 ℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Alkaline phosphatase is highly purified from calf intestine.
Alkaline phosphatase (ALP) removes phosphate groups from the 5′ end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays.

碱性磷酸酶(ALP或AKP)是广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织经肝脏向胆外排出的一种酶。
在免疫学研究方面, 已广泛应用AP 标记抗体进行酶联免疫荧光反应(E LISA)和Western 印迹分析, 即将A P 与显色剂或去磷酸化后能发光的底物相互作用来揭示靶与检测酶复合物的存在, 与辣根过氧化物酶相比, AP 用作标记酶的优点是稳定性高、灵敏度高, 缺点是成本高、标记困难。在生物化学和分子生物学方面, 用AP 催化除去DNA 分子的5′末端磷酸基团以防止载体自连是基因克隆中的常规手段之一。

碱性磷酸酶(来源于牛肠粘膜,进口)

上海金畔生物科技有限公司提供碱性磷酸酶(来源于牛肠粘膜,进口) ,欢迎访问官网了解更多产品信息。

产品编号 D-9409
英文名称 Alkaline Phosphatase(≥10u/mg) Protein
中文名称 碱性磷酸酶(来源于牛肠粘膜,进口)
别    名 Phosphatase, Alkaline from bovine intestinal mucosa; AP; ALP; Alkaline Phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum);   碱性磷酸酶
保存条件 Store at -20 ℃.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Alkaline phosphatase is highly purified from calf intestine.
Alkaline phosphatase (ALP) removes phosphate groups from the 5′ end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays.

基本信息:
CAS:9001-78-9
分子量:dimer ~160 kDa
MDL:MFCD00131849
外观:冻干粉末
来源:牛小肠黏膜
比活性:≥10 DEA units/mg solid

产品简介:
碱性磷酸酶(ALP或AKP)是广泛分布于人体肝脏、骨骼、肠、肾和胎盘等组织经肝脏向胆外排出的一种酶。
在免疫学研究方面, 已广泛应用AP 标记抗体进行酶联免疫荧光反应(E LISA)和Western 印迹分析, 即将A P 与显色剂或去磷酸化后能发光的底物相互作用来揭示靶与检测酶复合物的存在, 与辣根过氧化物酶相比, AP 用作标记酶的优点是稳定性高、灵敏度高, 缺点是成本高、标记困难。在生物化学和分子生物学方面, 用AP 催化除去DNA 分子的5′末端磷酸基团以防止载体自连是基因克隆中的常规手段之一。