标签归档：buffers

T4 DNA Polymerase | NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 DNA Polymerase

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. 

  • Gap filling (no strand displacement activity)
  • Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
  • Lacks 5’ —> 3’ exonuclease activity
  • Probe labeling using replacement synthesis
  • Singe-strand deletion subcloning

精选视频

T4 DNA Polymerase | NEB酶试剂 New England Biolabs

DNA Blunting Tutorial

观看其他视频

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0203S 3,000 units/ml 150 units ¥749.00 有 有 有 有 有
M0203L 3,000 units/ml 750 units ¥2,979.00 有 有 有 无 有
如何订购
Please enter a quantity for at least one size

T4 DNA Polymerase | NEB酶试剂 New England Biolabs Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

产品信息

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli).  Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

产品来源

Purified from a strain of E. coli that carries the T4 DNA Polymerase gene.

产品类别:
DNA Manipulation Products

应用:
Blunting,
Polymerases for DNA Manipulation,
PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0203S     -20    
        T4 DNA Polymerase M0203SVIAL -20 1 x 0.05 ml 3,000 units/ml
        NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X
    • M0203L     -20    
        T4 DNA Polymerase M0203LVIAL -20 1 x 0.25 ml 3,000 units/ml
        NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C (5).

    反应条件

    1X NEBuffer™ r2.1

    1X NEBuffer™ r2.1
    50 mM NaCl
    10 mM Tris-HCl
    10 mM MgCl2
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ 1: 60%
    NEBuffer™ 2: 100%
    NEBuffer™ 3: 100%
    NEBuffer™ 4: 100%

    贮存溶液

    100 mM KPO4
    1 mM DTT
    50% Glycerol
    pH 6.5 @ 25°C

    热失活

    75°C for 20 min

    分子量

    理论上的: 104000 daltons

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    No

    单位活性检测条件

    1X NEBuffer 2.1, 33 µM dNTPs including [3H]-dTTP, 70 µg/ml denatured herring sperm DNA.

    错配率

    ~ 1×10-6bases

  • 优势和特性

    应用特性

    • 3´ overhang removal to form blunt ends (1,2).
    • 5´ overhang fill-in to form blunt ends (1,2).
    • Single strand deletion subcloning (3).
    • Second strand synthesis in site-directed mutagenesis (4).
    • Probe labeling using replacement synthesis (1,2).

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • BSA，分子生物学级
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • NEBuffer™ r2.1

  • 注意事项
    1. For fill-in reactions only: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, 3.1 and CutSmart® Buffer as well as NEBuffers 1-4 and T4 DNA Ligase Reaction Buffer.
    2. For blunting reactions requiring removal of overhangs: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, and CutSmart Buffer as well as NEBuffers 1, 2, and 4 and T4 DNA Ligase Reaction Buffer. NEBuffers 3.1 and 3 are not recommended when overhang removal is required.
    3. Optimal activity is observed in NEBuffer 2.1.
    4. Supplement with dNTPs.*
    5. BSA supplementation is recommended when using a buffer that does not already contain BSA.
    6. Incubate at temperature suggested for specific protocol.

      * Refer to specific protocol to determine recommended dNTP concentrations.

      Refer

  • 参考文献
    1. Tabor, S. and Struhl, K. (1989). DNA-Dependent DNA Polymerases. In F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl(Ed.), Current Protocols in Molecular Biology. 3.5.10-3.5.12. New York: John Wiley & Sons, Inc.
    2. Sambrook, J. et al. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), 5.44-5.47. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
    3. Dale, R. et al. (1985). Plasmid. 13, 31-40.
    4. Kunkel, T.A. et al. (1987). Methods Enzymol.. 154, 367-382.
    5. Panet, A. et al. (1973). Biochemistry. 12, 5045-5050.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for blunting ends by 3’ overhang removal and 3’ recessed (5’ overhang) end fill-in using T4 DNA Polymerase (M0203)

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • Blunting Selection Chart
    • Common Applications for Exonucleases and Endonucleases
    • DNA Polymerase Selection Chart

  • Web 工具
    • NEBcloner®

FAQs & 问题解决指南

  • FAQs
    1. Can T4 DNA Polymerase be used in other NEBuffers and rCutSmart Buffer?
    2. Can T4 DNA Polymerase be used to blunt DNA?
    3. Can T4 DNA Polymerase be used to fill in 3′ overhangs?
    4. Can T4 DNA Polymerase be used to remove 5′ overhangs?
    5. Can T4 DNA Polymerase be heat inactivated?
    6. Are the nucleotides needed to remove a 3′ overhang using T4 DNA Polymerase?
    7. What are the main causes of blunting reaction failure using T4 DNA Polymerase?
    8. Can T4 DNA Polymerase be used in labeling reactions and partial fill in reactions?
    9. Is T4 DNA Polymerase active at room temperature?
    10. Is T4 DNA Polymerase the enzyme of choice for removing 3′ overhangs and filling in 5′ overhangs (3′ recessed ends)?
    11. Are NEB DNA Polymerases supplied with dNTPs?
    12. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

  • 实验技巧
    Excess enzyme, temperatures above 12°C, or limited dNTP concentrations can cause excessive 3’ → 5’ exonuclease degradation, which eliminates blunt end formation.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    T4 DNA Polymerase | NEB酶试剂 New England Biolabs Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0203S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0203S_L_v1_0401512
    • M0203S_L_v1_0401606
    • M0203S_L_v1_0401612
    • M0203S_L_v1_0401706
    • M0203S_L_v1_0401712
    • M0203S_v1_10011307
    • M0203S_v1_10021832
    • M0203L_v1_10019934
    • M0203L_v1_10023074
    • M0203S_v1_10029314
    • M0203L_v1_10034499
    • M0203S_v1_10034498
    • M0203L_v1_10036401
    • M0203L_v1_10043962
    • M0203S_v1_10043963
    • M0203L_v1_10048109
    • M0203S_v1_10049314
    • M0203S_v1_10054179
    • M0203L_v1_10055853
    • M0203S_v1_10055855
    • M0203L_v1_10060120
    • M0203S_v1_10060122
    • M0203L_v1_10063501
    • M0203S_v1_10063553
    • M0203L_v1_10068639
    • M0203S_v1_10070264
    • M0203L_v1_10074951
    • M0203S_v1_10075027
    • M0203S_v1_10081947
    • M0203S_v1_10085012
    • M0203L_v1_10085011
    • M0203S_v1_10091124
    • M0203S_v1_10091904
    • M0203L_v1_10091907
    • M0203L_v1_10099938
    • M0203S_v1_10099937
    • M0203L_v1_10107643
    • M0203S_v1_10107644
    • M0203S_v1_10112939
    • M0203L_v1_10113492
    • M0203L_v1_10127968
    • M0203S_v1_10127969
    • M0203S_v1_10143141
    • M0203L_v1_10145960
    • M0203L_v1_10151704
    • M0203S_v1_10151890

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • T4 DNA Polymerase
    • NEBuffer™ r2.1

T4 DNA Polymerase |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 DNA Polymerase

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. 

  • Gap filling (no strand displacement activity)
  • Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
  • Lacks 5’ —> 3’ exonuclease activity
  • Probe labeling using replacement synthesis
  • Singe-strand deletion subcloning

精选视频

T4 DNA Polymerase |

DNA Blunting Tutorial

观看其他视频

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0203S 3,000 units/ml 150 units ¥749.00 有 有 有 有 有
M0203L 3,000 units/ml 750 units ¥2,979.00 有 有 有 无 有
如何订购
Please enter a quantity for at least one size

T4 DNA Polymerase | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

产品信息

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. This enzyme has a 3´→ 5´ exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli).  Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5´→ 3´ exonuclease function.

产品来源

Purified from a strain of E. coli that carries the T4 DNA Polymerase gene.

产品类别:
DNA Manipulation Products

应用:
Blunting,
Polymerases for DNA Manipulation,
PCR

  • 产品组分信息

    产品组分信息

    本产品提供以下试剂或组分：

    NEB # 名称 组分货号 储存温度 数量 浓度
    • M0203S     -20    
        T4 DNA Polymerase M0203SVIAL -20 1 x 0.05 ml 3,000 units/ml
        NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X
    • M0203L     -20    
        T4 DNA Polymerase M0203LVIAL -20 1 x 0.25 ml 3,000 units/ml
        NEBuffer™ r2.1 B6002SVIAL -20 1 x 1.25 ml 10 X

  • 特性和用法

    单位定义

    One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C (5).

    反应条件

    1X NEBuffer™ r2.1

    1X NEBuffer™ r2.1
    50 mM NaCl
    10 mM Tris-HCl
    10 mM MgCl2
    100 µg/ml Recombinant Albumin
    (pH 7.9 @ 25°C)

    在不同缓冲液中的活性

    NEBuffer™ 1: 60%
    NEBuffer™ 2: 100%
    NEBuffer™ 3: 100%
    NEBuffer™ 4: 100%

    贮存溶液

    100 mM KPO4
    1 mM DTT
    50% Glycerol
    pH 6.5 @ 25°C

    热失活

    75°C for 20 min

    分子量

    理论上的: 104000 daltons

    5′ – 3′ 核酸外切酶

    No

    3′ – 5′ 核酸外切酶

    Yes

    链置换

    No

    单位活性检测条件

    1X NEBuffer 2.1, 33 µM dNTPs including [3H]-dTTP, 70 µg/ml denatured herring sperm DNA.

    错配率

    ~ 1×10-6bases

  • 优势和特性

    应用特性

    • 3´ overhang removal to form blunt ends (1,2).
    • 5´ overhang fill-in to form blunt ends (1,2).
    • Single strand deletion subcloning (3).
    • Second strand synthesis in site-directed mutagenesis (4).
    • Probe labeling using replacement synthesis (1,2).

  • 相关产品

    相关产品

    • Deoxynucleotide (dNTP) Solution Set
    • Deoxynucleotide (dNTP) Solution Mix
    • BSA，分子生物学级
    • Monarch® Plasmid Miniprep Kit 
    • Monarch® DNA Gel Extraction Kit
    • Monarch® PCR & DNA Cleanup Kit (5 μg)

    单独销售的组分

    • NEBuffer™ r2.1

  • 注意事项
    1. For fill-in reactions only: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, 3.1 and CutSmart® Buffer as well as NEBuffers 1-4 and T4 DNA Ligase Reaction Buffer.
    2. For blunting reactions requiring removal of overhangs: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, and CutSmart Buffer as well as NEBuffers 1, 2, and 4 and T4 DNA Ligase Reaction Buffer. NEBuffers 3.1 and 3 are not recommended when overhang removal is required.
    3. Optimal activity is observed in NEBuffer 2.1.
    4. Supplement with dNTPs.*
    5. BSA supplementation is recommended when using a buffer that does not already contain BSA.
    6. Incubate at temperature suggested for specific protocol.

      * Refer to specific protocol to determine recommended dNTP concentrations.

      Refer

  • 参考文献
    1. Tabor, S. and Struhl, K. (1989). DNA-Dependent DNA Polymerases. In F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl(Ed.), Current Protocols in Molecular Biology. 3.5.10-3.5.12. New York: John Wiley & Sons, Inc.
    2. Sambrook, J. et al. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), 5.44-5.47. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
    3. Dale, R. et al. (1985). Plasmid. 13, 31-40.
    4. Kunkel, T.A. et al. (1987). Methods Enzymol.. 154, 367-382.
    5. Panet, A. et al. (1973). Biochemistry. 12, 5045-5050.

操作说明、说明书 & 用法

  • 操作说明
    1. Protocol for blunting ends by 3’ overhang removal and 3’ recessed (5’ overhang) end fill-in using T4 DNA Polymerase (M0203)

  • 使用指南
    • Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

工具 & 资源

  • 选择指南
    • Blunting Selection Chart
    • Common Applications for Exonucleases and Endonucleases
    • DNA Polymerase Selection Chart

  • Web 工具
    • NEBcloner®

FAQs & 问题解决指南

  • FAQs
    1. Can T4 DNA Polymerase be used in other NEBuffers and rCutSmart Buffer?
    2. Can T4 DNA Polymerase be used to blunt DNA?
    3. Can T4 DNA Polymerase be used to fill in 3′ overhangs?
    4. Can T4 DNA Polymerase be used to remove 5′ overhangs?
    5. Can T4 DNA Polymerase be heat inactivated?
    6. Are the nucleotides needed to remove a 3′ overhang using T4 DNA Polymerase?
    7. What are the main causes of blunting reaction failure using T4 DNA Polymerase?
    8. Can T4 DNA Polymerase be used in labeling reactions and partial fill in reactions?
    9. Is T4 DNA Polymerase active at room temperature?
    10. Is T4 DNA Polymerase the enzyme of choice for removing 3′ overhangs and filling in 5′ overhangs (3′ recessed ends)?
    11. Are NEB DNA Polymerases supplied with dNTPs?
    12. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

  • 实验技巧
    Excess enzyme, temperatures above 12°C, or limited dNTP concentrations can cause excessive 3’ → 5’ exonuclease degradation, which eliminates blunt end formation.

引用 & 技术文献

  • 引用文献

    产品引用文献查找工具

    T4 DNA Polymerase | Powered by Bioz See more details on Bioz

质控、安全 & 法规

  • 质控分析
    每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。

  • 产品说明与变更通知

    产品说明与变更通知

    产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]

    • M0203S_L_v1

  • CoA
    CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]

    • M0203S_L_v1_0401512
    • M0203S_L_v1_0401606
    • M0203S_L_v1_0401612
    • M0203S_L_v1_0401706
    • M0203S_L_v1_0401712
    • M0203S_v1_10011307
    • M0203S_v1_10021832
    • M0203L_v1_10019934
    • M0203L_v1_10023074
    • M0203S_v1_10029314
    • M0203L_v1_10034499
    • M0203S_v1_10034498
    • M0203L_v1_10036401
    • M0203L_v1_10043962
    • M0203S_v1_10043963
    • M0203L_v1_10048109
    • M0203S_v1_10049314
    • M0203S_v1_10054179
    • M0203L_v1_10055853
    • M0203S_v1_10055855
    • M0203L_v1_10060120
    • M0203S_v1_10060122
    • M0203L_v1_10063501
    • M0203S_v1_10063553
    • M0203L_v1_10068639
    • M0203S_v1_10070264
    • M0203L_v1_10074951
    • M0203S_v1_10075027
    • M0203S_v1_10081947
    • M0203S_v1_10085012
    • M0203L_v1_10085011
    • M0203S_v1_10091124
    • M0203S_v1_10091904
    • M0203L_v1_10091907
    • M0203L_v1_10099938
    • M0203S_v1_10099937
    • M0203L_v1_10107643
    • M0203S_v1_10107644
    • M0203S_v1_10112939
    • M0203L_v1_10113492
    • M0203L_v1_10127968
    • M0203S_v1_10127969
    • M0203S_v1_10143141
    • M0203L_v1_10145960
    • M0203L_v1_10151704
    • M0203S_v1_10151890

  • SDS
    以下 SDS 文件可以帮助您安全地使用该产品

    • T4 DNA Polymerase
    • NEBuffer™ r2.1

BioGenes BlueCap Buffers说明书

发表于由

 

BioGenes是定制免疫测定和抗体开发的服务提供商，致力于质量和服务。

BioGenes成立于1992年，总部位于德国柏林，是一家*的合作伙伴，为40个国家的600多家客户提供服务。该公司与制药，生物技术公司，CMO和体外诊断公司保持着长期合作关系。

BioGenes BlueCap Buffers说明书

BlueCap Buffers

 

BLUECAP LX-BUFFER

 

准备使用的
pH为7.2±0.2 
含有0.05％的Proclin ®  300

保质期在-20°C：1年 – 反复冷冻和解冻循环可能
在2至8°C的保质期：6个月

仅用于体外研究。

产品产品代码

BlueCap LX-Buffer

S 201

€52.00

 *

BlueCap封锁解决方案

S 204

€52.00

 *

BlueCap抗体稳定剂基于TRIS

S208

€48.00

 *

BlueCap洗涤缓冲液Tris不含Tween 500ml

S 211.500

€36.00

 *

BlueCap POD稳定剂

S 220

€31.00

 *

 

大肠杆菌| 360-HCP ELISA试剂盒和组件

大肠杆菌| 360-HCP ELISA是增强的通用宿主细胞蛋白（HCP）测定。它被设计为涵盖比其他商业上可获得的通用HCP测定更广泛的大肠杆菌宿主细胞蛋白（HCP）。

 

大肠杆菌| 360-HCP ELISA基于W3110和BL21（DE3）细胞系，其在不同的培养基和条件下发酵，产生具有不同HCP模式的几种抗原制剂。通过用这些抗原免疫山羊，BioGenes开发了一组四种不同的HCP ELISA（A到D），它们共同构建了增强的通用大肠杆菌| 360-HCP ELISA。

 

通过覆盖相应大肠杆菌| 360-HCP抗原分布的≥80％证明了所有四种抗体制剂（A至D）的高特异性。通过使用Cy5标记的大肠杆菌| 360-HCP标准品的2D Western印迹和相应的Cy3标记的抗HCP抗体测量抗原覆盖度。

 

在接受标准100±30％的加标抗原内，在测定工作范围内的加标样品中估计满意的恢复。通过验证参数LOD，LOQ和工作范围描述的大肠杆菌| 360-HCP ELISA试剂盒（A至D）的高灵敏度如下所示：

 

LOD：0.2-0.5ng / mL

LOQ：0.6 – 1.6 ng / mL

工作范围：2 – 100 ng / mL

 

矿物介质 LB中等

A型：BL21（DE3） D型：BL21（DE3）

B型：W3110 C型：W3110

产品产品代码E.coli | 360 HCP ELISA Type AD Starter Set

IP 2001 N / A

E.coli | 360 HCP ELISA BL21（DE3）Starter Set

IP 2002 N / A

E.coli | 360-HCP ELISA W3110 Starter Set

IP 2003 N / A

大肠杆菌| 360-HCP ELISA A型

IP 201 N / A

大肠杆菌| 360-HCP ELISA B型

IP 202 N / A

大肠杆菌| 360-HCP ELISA C型

IP 203 N / A

大肠杆菌| 360-HCP ELISA D型

IP 204 N / A

抗大肠杆菌抗体A型

IP 211 N / A

抗大肠杆菌抗体B型

IP 212 N / A

抗大肠杆菌抗体C型

IP 213 N / A

抗大肠杆菌抗体D型

IP 214 N / A

大肠杆菌| 360-HCP标准A型

IP 221 N / A

大肠杆菌| 360-HCP标准B型

IP 222 N / A

大肠杆菌| 360-HCP标准C型

IP 223 N / A

大肠杆菌| 360-HCP标准型D.

IP 224 N / A

大肠杆菌| 360-HCP分析缓冲液

IP 230 N / A

 

Product No.

Product Name

Price

Starter Sets

 

 

IP 2001

E.coli 360 HCP ELISA Starter Set Type A , B, C and D

1.335,00 EUR

IP 2002

E.coli 360 HCP ELISA BL21 Starter Set Type A + D

668,00 EUR

IP 2003

E.coli 360 HCP ELISA W3110 Starter Set Type B + C

668,00 EUR

 

 

 

Single Kits

 

 

IP 201

E.coli 360 HCP ELISA Type A

445,00 EUR

IP 202

E.coli 360 HCP ELISA Type B

445,00 EUR

IP 203

E.coli 360 HCP ELISA Type C

445,00 EUR

IP 204

E.coli 360 HCP ELISA Type D

445,00 EUR

 

 

 

Antibodies

 

 

IP 211

Anti-E.coli antibody, Type A

405,00 EUR

IP 212

Anti-E.coli antibody, Type B

405,00 EUR

IP 213

Anti-E.coli antibody, Type C

405,00 EUR

IP 214

Anti-E.coli antibody, Type D

405,00 EUR

 

 

 

Standards

 

 

IP 221

E.coli-HCP Standard Type A

1.080,00 EUR

IP 222

E.coli-HCP Standard Type B

1.080,00 EUR

IP 223

E.coli-HCP Standard Type C

1.080,00 EUR

IP 224

E.coli-HCP Standard Type D

1.080,00 EUR

 

 

 

Buffer

 

 

IP 230

Assay Buffer (1×100 mL)

45,00 EUR

 

 

 

 

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K. lactis Protein Expression Kit |NEB酶试剂 New England Biolabs

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K. lactis Protein Expression Kit

The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.

  • Supplied with competent K. lactis cells (NEB# C1001)
  • Allows cloning and expression of genes toxic to E. coli
  • Rapid high cell density growth results in high yield protein expression 
  • Easy-to-use protocols for those inexperienced with yeast systems
  • No expensive antibiotics or methanol required
  • Attractive commercial sub-licensing