上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
产品信息
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Comparison of OneTaq Products to Other Commercially Available Polymerases
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent® DNA Polymerase gene from Pyrococcus species GB-D.
PCR
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||
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20 mM Tris-HCl
22 mM KCl
22 mM NH4Cl
1.8 mM MgCl2
5% Glycerol
0.05% Tween® 20
0.06% IGEPAL® CA-630
0.2 mM dNTPs
25 units/ml OneTaq® DNA Polymerase
pH 8.9 @ 25°C
否
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
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产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
1. Josh Quick 2020. nCoV-2019 sequencing protocol v3 (LoCost). protocols.io
Want to try this product? Request a sample 
Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
LunaScript RT SuperMix is an optimized master mix for first strand cDNA synthesis and can be used in amplicon sequencing or a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, LunaScript RT SuperMix contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The product offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
For RT-PCR, RNA-seq, and other applications, LunaScript RT Master Mix Kit (Primer-free) (NEB #E3025) is an optimized master mix containing all the necessary components for first strand cDNA synthesis, except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis flexibility. Learn more here.
In comparison to the LunaScript kit version (NEB #E3010), this LunaScript product does not contain a No-RT Control Mix or nuclease-free water. More details can be found below:
A. A serial dilution of Jurkat total RNA (1 μg – 1 pg) was converted to cDNA and then quantitated by qPCR using a β-actin target.
B. ERCC (External RNA Controls Consortium) mix1 RNA containing 5×109 to 50 copies of ERCC00130 (~10 ng – 10 fg) was converted to cDNA and then quantitated by qPCR.
Learn more about this “Dots in Boxes” visualization method.
Learn more about this “Dots in Boxes” visualization method.
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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操作说明、说明书 & 用法
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
This product will be discontinued on 12/15/2021 and replaced with NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™.
产品信息
New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
100 µg/ml BSA
pH [email protected]°C
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
This product will be discontinued on 12/15/2021 and replaced with NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™.
产品信息
New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
10 mM Bis-Tris-Propane-HCl
10 mM MgCl2
100 µg/ml BSA
pH [email protected]°C
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
This product was discontinued on 12/15/2021 and replaced with NEBuffer r2.1.
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity.
Most of our enzymes are supplied with one of four standard NEBuffers. Visit NEBCutSmart.com for details.
产品信息
New England Biolabs provides a colorcoded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml BSA
pH [email protected]°C
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
