Instant Sticky-end Ligase Master Mix will ligate these substrates:

Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.

• No incubation time necessary to achieve ligation
• No thawing of the master mix is required as liquid state is maintained when stored at -20°C
• Simplified reaction setup with optimized ratio of enzyme and buffer components
• Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
• Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4, and Blunt/TA Ligase Master Mix
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

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Quick Tips – Do I need a 5′ phosphate for ligation?