Hi-T4 DNA Ligase will ligate these substrates:

• Hi-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved thermostability compared to T4 DNA Ligase
• Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids (1)
• Supplied with both T4 DNA Ligase Buffer and StickTogether^™ DNA Ligase Buffer
• Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Instant Sticky-end Ligase Master Mix and Blunt/TA Ligase Master Mix
• This product can be purchased in larger volumes. Submit an inquiry to find out more.
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

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DNA Ligation

• Immobilized T4 DNA Ligase (IM T4 DNA Ligase) consists of T4 DNA Ligase covalently bound to magnetic beads. 
• Easily remove enzyme from reaction using a magnet 
• Avoid heat inactivation step, which may interfere with downstream applications
• Re-use immobilized enzymes 
• Enable new workflows and applications

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Learn about Immobilized T4 DNA Ligase

• T3 DNA Ligase is an ATP-dependent dsDNA Ligase from bacteriophage T3
• 2x salt tolerence as compared to T4 DNA Ligase
• This product can be purchased in larger volumes. Submit an inquiry to find out more.
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

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DNA Ligation

T4 DNA Ligase will ligate these substrates:

• Catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
• This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids (1).
• The industry standard for performance and quality
• Applicable for use in high-complexity library cloning
• Other T4 DNA Ligase products include Quick Ligation Kit, Salt-T4, Hi-T4, Instant Sticky-end Ligase Master Mix and Blunt/TA Ligase Master Mix
• This product can be purchased in larger volumes. Submit an inquiry to find out more.
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

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Quick Tips – Do I need a 5′ phosphate for ligation?

T7 DNA Ligase is an ATP-dependent dsDNA ligase from bacteriophage T7.

• Ligates sticky-ends only 
• This product can be purchased in larger volumes. Submit an inquiry to find out more.
• Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart

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DNA Ligation