标签归档：his

SUMO蛋白酶 (His tag)

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上海金畔生物科技有限公司提供SUMO蛋白酶 (His tag) ，欢迎访问官网了解更多产品信息。

产品编号 D13109
英文名称 SUMO Protease (His tag)
中文名称 SUMO蛋白酶 (His tag)
别    名 SUMO Protease (His tagged recombinant protein); Small Ubiquitin-like Modifier Protease; ULP; Ubiquitin like protease; Ubiquitin-homology domain protein PIC1; Ubl-specific protease 1; recombinant protein, His tagged; Small Ubiquitin-related Modifier Protease;   泛素样修饰的一种小蛋白 (SUMO);
保存条件 长期储存于-80℃，或解冻后保存于-20℃。有效期1年。
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 分子量：27 kDa
级别：BR
纯度：≥95% (SDS-PAGE)
酶活：5KU/ml
外观/性状：液体
适用pH：6.0-8.0 最佳： 7.0
来源：Expressed in E. coli (His tagged recombinant protein)
酶活定义：一个酶单位的定义是在30°C下1小时内可减少90%的SUMO-GST。
介绍：SUMO蛋白酶 (Ulp1) 是一类能特异性去除翻译后蛋白修饰（PTM）的酶，被称为小泛素相关修饰（SUMO），属于泛素和/或泛素样蛋白（UBL）的PTM类。通常被称为“SUMO蛋白酶”的酶是来自酿酒酵母的Ubl特异性蛋白酶1（Ulp1）。是切割小分子泛素修饰(SUMO)融合蛋白获得天然N端靶蛋白的一种工具酶，具有酶切效率高，特异性好等优点。SUMO蛋白酶适应范围广，在温度范围（2–30°C）、离子强度（0–400 mM NaCl）和pH范围（5.5-9.5）条件下仍具有活性。然而，它的活性可能因底物和条件而异。研究人员需要优化他们的具体反应条件。本产品是基因工程重组蛋白，它能识别 SUMO 蛋白的三级结构，而不是氨基酸序列，因此可以高效而且特异性地将 SUMO 蛋白从重组融合蛋白上切割下来。SUMO 蛋白酶带有 His 标签，便于融合蛋白切割后利用亲和层析去除该蛋白酶。
推荐参考条件：每毫克目标蛋白可使用20单位的SUMO蛋白酶，在30°C下使用1小时，或在2–8°C下过夜。然后可通过SDS-PAGE评估切割效率。如有必要，可以调整SUMO蛋白酶的用量。SUMO蛋白酶在还原剂（如0.5–2 mM DTT）存在下工作得更好。反应混合物中的DTT能显著提高裂解效率，尤其是在较长的培养时间内。

pEF1/myc-His C载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPI0028 pEF1/myc-His C Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pEF1/myc-His B载体说明书

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型号 载体名称 出品公司 载体用途 价格
VPI0027 pEF1/myc-His B Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pEF1/myc-His A载体说明书

发表于由

型号 载体名称 出品公司 载体用途 价格
VPI0026 pEF1/myc-His A Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pUB6/V5 His C说明书

发表于由

 

 pUB6/V5 His C

型号 载体名称 出品公司 载体用途
VPI0024 pUB6/V5 His C Invitrogen 哺乳动物表达载体

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: