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RecJf
- DNA specific exonuclease
- Catalyzes the removal of nucleotides from linear single-stranded DNA in the 5′ to 3′ direction
- Preferred substrate is double-stranded DNA with 5′ single stranded overhangs > 6 nucleotides long
RecJf can be used for:
- Degradation of single-stranded DNA from the 5′-end
- Removal of 5′ protruding single-stranded termini at the ends of linear double-stranded DNA (blunt ends are not exclusively created)
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